Detailed information of TA system TA115356

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2751079..2752667
• Replicon: chromosome
• Accession: NZ_CP034136
• Organism: Klebsiella quasipneumoniae subsp. similipneumoniae strain G747

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: BME36_RS14025
• Protein ID: WP_064187239.1
• Coordinates: 2751079..2752401 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2A5MFC9
• Locus tag: BME36_RS14030
• Protein ID: WP_004205921.1
• Coordinates: 2752401..2752667 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BME36_RS13985	2746161..2746379	+	219	WP_002904808.1	ParD-like family protein	-
BME36_RS13990	2746360..2747142	+	783	WP_048294851.1	type I methionyl aminopeptidase	-
BME36_RS13995	2747411..2748526	+	1116	WP_064177840.1	serine hydrolase	-
BME36_RS14000	2748513..2748857	+	345	WP_004205915.1	hypothetical protein	-
BME36_RS14005	2748886..2749419	+	534	WP_053810394.1	hypothetical protein	-
BME36_RS14010	2749422..2749628	+	207	WP_004205917.1	helix-turn-helix transcriptional regulator	-
BME36_RS14020	2749770..2751062	+	1293	WP_023318214.1	glycoside hydrolase family 10 protein	-
BME36_RS14025	2751079..2752401	-	1323	WP_064187239.1	type II toxin-antitoxin system HipA family toxin	Toxin
BME36_RS14030	2752401..2752667	-	267	WP_004205921.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
BME36_RS14035	2752950..2753276	-	327	WP_053810395.1	hydroxyisourate hydrolase	-
BME36_RS14040	2753273..2753773	-	501	WP_017900378.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
BME36_RS14045	2753751..2755130	-	1380	WP_017900377.1	purine permease	-
BME36_RS14050	2755476..2756630	+	1155	WP_087812562.1	FAD-dependent urate hydroxylase HpxO	-
BME36_RS14055	2756611..2757534	-	924	WP_004205929.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mgtC / iroE	2573211..2801536	228325

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49026.36 Da
• Isoelectric Point: 8.6091

>T115356 WP_064187239.1 NZ_CP034136:c2752401-2751079 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MATLTTWMNNARVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRERIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLAALRWQTLDEAQLTALLTAYQSDIPLGMVTGQDDFRISVAGAQEKTAL
LRMGEHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARALGLAVPEAEIINTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILGEFADRVP
QAIESARRTLPSDFSAHVWRAITENMLKLHARLQQGLLAG

Nucleotide

• Length: 1323 bp

>T115356 NZ_CP034136:c2752401-2751079 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGGCGACGCTCACCACCTGGATGAACAATGCGCGCGTGGGAACCTTAACCCGCCAGGCCAACGGGGCGCACAGTTTCCG
CTATGATGAGGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCGCTGCCGCTGCAGTATGGCAATATTACCG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGAGCGGATCGTCAGGCGCTATCAG
GCGCGTTCGAAACAGCCGTTCGATCTGCTGGCCGAGGTCGGGCGGGACAGCGTAGGGGCGGTGACTCTGCTGCCGCCGGG
GGAAGAGGCGCATCTTGCCGCGTTGCGCTGGCAGACGCTGGATGAGGCGCAGCTGACCGCTTTGCTGACCGCCTACCAGT
CGGATATTCCTCTCGGGATGGTGACCGGCCAGGACGATTTTCGGATCTCGGTGGCCGGCGCTCAGGAGAAAACCGCGCTG
CTGCGAATGGGCGAGCACTGGTGTATTCCGCAGGGCACCACCCCGACCACCCATATCATTAAACTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAACGAATATCTGTGCCTGGCGCTGGCCAGGGCGTTGG
GCCTGGCGGTGCCGGAGGCGGAGATTATCAACACGCCGCGGATCCGTGCGCTGGCGGTGACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGGCGCGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCATTCGGCATACCGTCGTCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCCAGTGAGGCCCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTGTTCCAGTGGCTGACCGGGGCAACGGACGGGCATGCGAAGAACTTCTCGCTCTTTTTGCTG
CCCGGCGGCAGCTACCGGCTCACGCCGTTCTACGACATTATTTCGGCATTTCCGGTGCTGGGCGGGACGGGACTGCATCT
GCGCGACCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGGAAAACGGAGATTAACGCTATCTATCCGCGTCATT
TTCTGGCGACCGCCAAAGCGGTTAACTTCCCCCGGGAGCAGATGCTGGCGATCCTGGGCGAGTTTGCCGACCGCGTGCCG
CAGGCCATCGAAAGCGCCCGCCGGACGCTGCCGTCGGACTTTTCCGCCCACGTCTGGCGGGCCATCACGGAGAACATGCT
TAAACTGCACGCCCGCCTGCAGCAAGGGCTGCTGGCGGGATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9940.38 Da
• Isoelectric Point: 7.1997

>AT115356 WP_004205921.1 NZ_CP034136:c2752667-2752401 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQEALASRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKPAETAMIP
DNTTDLDW

Nucleotide

• Length: 267 bp

>AT115356 NZ_CP034136:c2752667-2752401 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGAATTCCCCGCAGATATACAGTCCGGTTCAGCTGGCCAATTACCTGAAACTGCTGCGCCAGCAAAATCACTGGACTCA
GGAGGCGCTGGCCAGCCGGATCGGCATTAAACAGGCGACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACCCTGTTTAAGATCCTCCAGTCGCTGGGAGTGGTGATGACCCTGAACGCCAAACCGGCAGAGACGGCGATGATTCCC
GATAACACCACGGATCTTGACTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2A5MFC9