Detailed information of TA system TA115346

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 53699..54342
• Replicon: plasmid pG4584_81.9Kb
• Accession: NZ_CP034132
• Organism: Klebsiella quasipneumoniae strain G4584

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: BME54_RS30135
• Protein ID: WP_087812770.1
• Coordinates: 53699..54115 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: BME54_RS30140
• Protein ID: WP_001261282.1
• Coordinates: 54112..54342 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BME54_RS30105	49007..49483	+	477	WP_000043177.1	DNA starvation/stationary phase protection protein	-
BME54_RS30110	49788..50138	-	351	WP_000928911.1	metalloregulator ArsR/SmtB family transcription factor	-
BME54_RS30115	50300..51958	+	1659	WP_000706606.1	FAD-dependent oxidoreductase	-
BME54_RS30120	52203..52667	+	465	WP_001243598.1	isoprenylcysteine carboxylmethyltransferase family protein	-
BME54_RS30125	52807..53511	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
BME54_RS30135	53699..54115	-	417	WP_087812770.1	type II toxin-antitoxin system VapC family toxin	Toxin
BME54_RS30140	54112..54342	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BME54_RS30145	54299..54711	+	413	Protein_58	hypothetical protein	-
BME54_RS30150	54883..55191	+	309	WP_048295720.1	hypothetical protein	-
BME54_RS30155	55227..55931	+	705	WP_087812771.1	hypothetical protein	-
BME54_RS30160	55931..56713	+	783	WP_032420986.1	site-specific integrase	-
BME54_RS30165	57338..58204	+	867	WP_032420987.1	replication initiation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	52807..53511	704
	-	inside	Non-Mobilizable plasmid	-	-	1..81935	81935

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15187.58 Da
• Isoelectric Point: 6.4640

>T115346 WP_087812770.1 NZ_CP034132:c54115-53699 [Klebsiella quasipneumoniae]
VNKIYMLDTNICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPDLVLEDWVR

Nucleotide

• Length: 417 bp

>T115346 NZ_CP034132:c54115-53699 [Klebsiella quasipneumoniae]
GTGAACAAGATCTATATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAGTTTGAGCGGGTGCCGGATCTGGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT115346 WP_001261282.1 NZ_CP034132:c54342-54112 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT115346 NZ_CP034132:c54342-54112 [Klebsiella quasipneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGAAGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3