Detailed information of TA system TA115345

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 64988..65513
• Replicon: plasmid pG4584_136.4Kb
• Accession: NZ_CP034131
• Organism: Klebsiella quasipneumoniae strain G4584

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: J5VBT8
• Locus tag: BME54_RS29335
• Protein ID: WP_004197633.1
• Coordinates: 65208..65513 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: BME54_RS29330
• Protein ID: WP_004197642.1
• Coordinates: 64988..65206 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BME54_RS29275	60094..61089	+	996	WP_019725651.1	hypothetical protein	-
BME54_RS29280	61162..61485	+	324	WP_019725650.1	hypothetical protein	-
BME54_RS31430	61919..62113	+	195	WP_019725033.1	hypothetical protein	-
BME54_RS29290	62157..62387	-	231	WP_019725034.1	hypothetical protein	-
BME54_RS29295	62401..62604	-	204	WP_019725040.1	hemolysin expression modulator Hha	-
BME54_RS29300	62638..63006	-	369	WP_015065502.1	hypothetical protein	-
BME54_RS29305	63050..63544	-	495	WP_009310053.1	hypothetical protein	-
BME54_RS29310	63575..64147	-	573	WP_015065504.1	hypothetical protein	-
BME54_RS29315	64144..64392	-	249	WP_019725036.1	hypothetical protein	-
BME54_RS29330	64988..65206	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
BME54_RS29335	65208..65513	+	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	Toxin
BME54_RS29340	65682..66083	+	402	WP_072199347.1	hypothetical protein	-
BME54_RS29345	66110..66433	+	324	WP_004197641.1	hypothetical protein	-
BME54_RS29350	66430..67446	+	1017	WP_004197639.1	hypothetical protein	-
BME54_RS29355	67644..68438	+	795	WP_004197635.1	site-specific integrase	-
BME54_RS29360	68886..69065	-	180	WP_006788217.1	hypothetical protein	-
BME54_RS29365	69185..69811	-	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA14 / qnrB1 / tet(A) / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaCTX-M-15 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2	-	1..136368	136368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11585.27 Da
• Isoelectric Point: 6.4661

>T115345 WP_004197633.1 NZ_CP034131:65208-65513 [Klebsiella quasipneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T115345 NZ_CP034131:65208-65513 [Klebsiella quasipneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT115345 WP_004197642.1 NZ_CP034131:64988-65206 [Klebsiella quasipneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT115345 NZ_CP034131:64988-65206 [Klebsiella quasipneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A2A5MCG5