Detailed information of TA system TA115344

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 120117..120853
• Replicon: plasmid pG4584_218.9Kb
• Accession: NZ_CP034130
• Organism: Klebsiella quasipneumoniae strain G4584

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: BME54_RS28305
• Protein ID: WP_003026803.1
• Coordinates: 120117..120599 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: BME54_RS28310
• Protein ID: WP_003026799.1
• Coordinates: 120587..120853 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BME54_RS28285	115254..116708	-	1455	WP_071841200.1	EAL domain-containing protein	-
BME54_RS28290	117311..117568	+	258	WP_023292103.1	hypothetical protein	-
BME54_RS28295	117852..118484	+	633	WP_001567369.1	hypothetical protein	-
BME54_RS28300	118513..119916	-	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
BME54_RS28305	120117..120599	-	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
BME54_RS28310	120587..120853	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
BME54_RS28320	121298..121528	-	231	WP_004206660.1	hypothetical protein	-
BME54_RS28325	121542..121745	-	204	WP_004206661.1	hemolysin expression modulator Hha	-
BME54_RS28330	121806..122297	-	492	WP_004206662.1	hypothetical protein	-
BME54_RS28335	122431..123642	-	1212	WP_032415728.1	hypothetical protein	-
BME54_RS28340	123639..123887	-	249	WP_004193994.1	hypothetical protein	-
BME54_RS28345	124245..124595	+	351	WP_004206664.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
BME54_RS28350	124647..125009	+	363	WP_004206665.1	arsenite efflux transporter metallochaperone ArsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	mrkJ / mrkF / mrkC / mrkB / mrkA	1..218935	218935
	-	flank	IS/Tn	-	-	118513..119916	1403

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T115344 WP_003026803.1 NZ_CP034130:c120599-120117 [Klebsiella quasipneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T115344 NZ_CP034130:c120599-120117 [Klebsiella quasipneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGCAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT115344 WP_003026799.1 NZ_CP034130:c120853-120587 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT115344 NZ_CP034130:c120853-120587 [Klebsiella quasipneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66