Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4162296..4162915 Replicon chromosome
Accession NZ_CP034129
Organism Klebsiella quasipneumoniae strain G4584

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag BME54_RS21300 Protein ID WP_002892050.1
Coordinates 4162697..4162915 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag BME54_RS21295 Protein ID WP_002892066.1
Coordinates 4162296..4162670 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BME54_RS21285 4157449..4158642 + 1194 WP_004204747.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
BME54_RS21290 4158665..4161811 + 3147 WP_004204748.1 multidrug efflux RND transporter permease subunit -
BME54_RS21295 4162296..4162670 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
BME54_RS21300 4162697..4162915 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
BME54_RS21305 4163073..4163639 + 567 WP_004204750.1 maltose O-acetyltransferase -
BME54_RS31250 4163611..4163733 - 123 WP_032426076.1 hypothetical protein -
BME54_RS21310 4163776..4164246 + 471 WP_049093338.1 YlaC family protein -
BME54_RS21315 4164215..4165672 - 1458 WP_049117056.1 PLP-dependent aminotransferase family protein -
BME54_RS21320 4165773..4166468 + 696 Protein_4100 GNAT family N-acetyltransferase -
BME54_RS21325 4166468..4166608 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
BME54_RS21330 4166608..4166871 - 264 WP_004204754.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T115340 WP_002892050.1 NZ_CP034129:4162697-4162915 [Klebsiella quasipneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T115340 NZ_CP034129:4162697-4162915 [Klebsiella quasipneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACTATGAATAAGCTGTACGATAAAATCCCGACGTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT115340 WP_002892066.1 NZ_CP034129:4162296-4162670 [Klebsiella quasipneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT115340 NZ_CP034129:4162296-4162670 [Klebsiella quasipneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACATTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References