Detailed information of TA system TA115339

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2802932..2804520
• Replicon: chromosome
• Accession: NZ_CP034129
• Organism: Klebsiella quasipneumoniae strain G4584

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: BME54_RS14390
• Protein ID: WP_064187239.1
• Coordinates: 2802932..2804254 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2A5MFC9
• Locus tag: BME54_RS14395
• Protein ID: WP_004205921.1
• Coordinates: 2804254..2804520 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BME54_RS14350	2798014..2798232	+	219	WP_002904808.1	ParD-like family protein	-
BME54_RS14355	2798213..2798995	+	783	WP_048294851.1	type I methionyl aminopeptidase	-
BME54_RS14360	2799264..2800379	+	1116	WP_064177840.1	serine hydrolase	-
BME54_RS14365	2800366..2800710	+	345	WP_004205915.1	hypothetical protein	-
BME54_RS14370	2800739..2801272	+	534	WP_053810394.1	hypothetical protein	-
BME54_RS14375	2801275..2801481	+	207	WP_004205917.1	helix-turn-helix transcriptional regulator	-
BME54_RS14385	2801623..2802915	+	1293	WP_023318214.1	glycoside hydrolase family 10 protein	-
BME54_RS14390	2802932..2804254	-	1323	WP_064187239.1	type II toxin-antitoxin system HipA family toxin	Toxin
BME54_RS14395	2804254..2804520	-	267	WP_004205921.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
BME54_RS14400	2804803..2805129	-	327	WP_053810395.1	hydroxyisourate hydrolase	-
BME54_RS14405	2805126..2805626	-	501	WP_017900378.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
BME54_RS14410	2805604..2806983	-	1380	WP_017900377.1	purine permease	-
BME54_RS14415	2807329..2808483	+	1155	WP_087812562.1	FAD-dependent urate hydroxylase HpxO	-
BME54_RS14420	2808464..2809387	-	924	WP_004205929.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mgtC / iroE	2627338..2853389	226051

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49026.36 Da
• Isoelectric Point: 8.6091

>T115339 WP_064187239.1 NZ_CP034129:c2804254-2802932 [Klebsiella quasipneumoniae]
MATLTTWMNNARVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRERIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLAALRWQTLDEAQLTALLTAYQSDIPLGMVTGQDDFRISVAGAQEKTAL
LRMGEHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARALGLAVPEAEIINTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILGEFADRVP
QAIESARRTLPSDFSAHVWRAITENMLKLHARLQQGLLAG

Nucleotide

• Length: 1323 bp

>T115339 NZ_CP034129:c2804254-2802932 [Klebsiella quasipneumoniae]
ATGGCGACGCTCACCACCTGGATGAACAATGCGCGCGTGGGAACCTTAACCCGCCAGGCCAACGGGGCGCACAGTTTCCG
CTATGATGAGGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCGCTGCCGCTGCAGTATGGCAATATTACCG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGAGCGGATCGTCAGGCGCTATCAG
GCGCGTTCGAAACAGCCGTTCGATCTGCTGGCCGAGGTCGGGCGGGACAGCGTAGGGGCGGTGACTCTGCTGCCGCCGGG
GGAAGAGGCGCATCTTGCCGCGTTGCGCTGGCAGACGCTGGATGAGGCGCAGCTGACCGCTTTGCTGACCGCCTACCAGT
CGGATATTCCTCTCGGGATGGTGACCGGCCAGGACGATTTTCGGATCTCGGTGGCCGGCGCTCAGGAGAAAACCGCGCTG
CTGCGAATGGGCGAGCACTGGTGTATTCCGCAGGGCACCACCCCGACCACCCATATCATTAAACTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAACGAATATCTGTGCCTGGCGCTGGCCAGGGCGTTGG
GCCTGGCGGTGCCGGAGGCGGAGATTATCAACACGCCGCGGATCCGTGCGCTGGCGGTGACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGGCGCGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCATTCGGCATACCGTCGTCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCCAGTGAGGCCCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTGTTCCAGTGGCTGACCGGGGCAACGGACGGGCATGCGAAGAACTTCTCGCTCTTTTTGCTG
CCCGGCGGCAGCTACCGGCTCACGCCGTTCTACGACATTATTTCGGCATTTCCGGTGCTGGGCGGGACGGGACTGCATCT
GCGCGACCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGGAAAACGGAGATTAACGCTATCTATCCGCGTCATT
TTCTGGCGACCGCCAAAGCGGTTAACTTCCCCCGGGAGCAGATGCTGGCGATCCTGGGCGAGTTTGCCGACCGCGTGCCG
CAGGCCATCGAAAGCGCCCGCCGGACGCTGCCGTCGGACTTTTCCGCCCACGTCTGGCGGGCCATCACGGAGAACATGCT
TAAACTGCACGCCCGCCTGCAGCAAGGGCTGCTGGCGGGATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9940.38 Da
• Isoelectric Point: 7.1997

>AT115339 WP_004205921.1 NZ_CP034129:c2804520-2804254 [Klebsiella quasipneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQEALASRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKPAETAMIP
DNTTDLDW

Nucleotide

• Length: 267 bp

>AT115339 NZ_CP034129:c2804520-2804254 [Klebsiella quasipneumoniae]
ATGAATTCCCCGCAGATATACAGTCCGGTTCAGCTGGCCAATTACCTGAAACTGCTGCGCCAGCAAAATCACTGGACTCA
GGAGGCGCTGGCCAGCCGGATCGGCATTAAACAGGCGACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACCCTGTTTAAGATCCTCCAGTCGCTGGGAGTGGTGATGACCCTGAACGCCAAACCGGCAGAGACGGCGATGATTCCC
GATAACACCACGGATCTTGACTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2A5MFC9