Detailed information of TA system TA115330

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 836270..836927
• Replicon: chromosome
• Accession: NZ_CP034129
• Organism: Klebsiella quasipneumoniae strain G4584

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: A0A8G2AFI7
• Locus tag: BME54_RS04305
• Protein ID: WP_064190060.1
• Coordinates: 836517..836927 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: W8UQ37
• Locus tag: BME54_RS04300
• Protein ID: WP_002916312.1
• Coordinates: 836270..836536 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BME54_RS04275	831427..832860	-	1434	WP_004205314.1	6-phospho-beta-glucosidase BglA	-
BME54_RS04280	832979..833707	-	729	WP_004205316.1	MurR/RpiR family transcriptional regulator	-
BME54_RS04285	833757..834068	+	312	WP_004144734.1	N(4)-acetylcytidine aminohydrolase	-
BME54_RS04290	834231..834890	+	660	WP_064190059.1	hemolysin III family protein	-
BME54_RS04295	835041..836024	-	984	WP_017899798.1	tRNA-modifying protein YgfZ	-
BME54_RS04300	836270..836536	+	267	WP_002916312.1	FAD assembly factor SdhE	Antitoxin
BME54_RS04305	836517..836927	+	411	WP_064190060.1	protein YgfX	Toxin
BME54_RS04310	836934..837455	-	522	WP_004205324.1	flavodoxin FldB	-
BME54_RS04315	837556..838452	+	897	WP_004144729.1	site-specific tyrosine recombinase XerD	-
BME54_RS04320	838475..839188	+	714	WP_004205326.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
BME54_RS04325	839194..840927	+	1734	WP_004205327.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16065.85 Da
• Isoelectric Point: 11.4778

>T115330 WP_064190060.1 NZ_CP034129:836517-836927 [Klebsiella quasipneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
DWEILGTPWVINSGMLLRLRHVDTRRSQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Nucleotide

• Length: 411 bp

>T115330 NZ_CP034129:836517-836927 [Klebsiella quasipneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCTCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGCTGGTTCCCTGGCCGTTAAGCTACACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTTGACTGTG
TGCGCAGCCAGCGACGGATCCATGCCCGTCGCGGCGAGATAAAACTGCTCACCGATTCCCGGCTGCGCTGGCAAAACGCC
GACTGGGAGATCCTCGGGACGCCGTGGGTCATCAACAGCGGAATGCTGCTGCGTTTGCGCCATGTCGATACCCGACGCAG
TCAGCATCTGTGGCTGGCGGCGGACAGCATGGATGCCGGAGAGTGGCGGGACCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10480.98 Da
• Isoelectric Point: 5.1116

>AT115330 WP_002916312.1 NZ_CP034129:836270-836536 [Klebsiella quasipneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT115330 NZ_CP034129:836270-836536 [Klebsiella quasipneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAACGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGAATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GY41