Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 62349..62992 Replicon plasmid p2b2
Accession NZ_CP034125
Organism Klebsiella pneumoniae strain BJCFK909

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag EH202_RS29290 Protein ID WP_001044770.1
Coordinates 62349..62765 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag EH202_RS29295 Protein ID WP_001261282.1
Coordinates 62762..62992 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EH202_RS29270 58452..58724 - 273 Protein_74 transposase -
EH202_RS29275 58753..59450 - 698 Protein_75 IS1 family transposase -
EH202_RS29280 59706..60728 - 1023 WP_000361404.1 helicase UvrD -
EH202_RS29285 60713..62275 - 1563 WP_004206609.1 AAA family ATPase -
EH202_RS29290 62349..62765 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
EH202_RS29295 62762..62992 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
EH202_RS29300 62949..63410 + 462 WP_164481824.1 hypothetical protein -
EH202_RS29305 63571..64515 + 945 WP_011977810.1 hypothetical protein -
EH202_RS29310 64552..64944 + 393 WP_011977811.1 hypothetical protein -
EH202_RS29315 65002..65523 + 522 WP_013214008.1 hypothetical protein -
EH202_RS29320 65569..65772 + 204 WP_011977813.1 hypothetical protein -
EH202_RS29325 65802..66806 + 1005 WP_011977814.1 hypothetical protein -
EH202_RS29330 66990..67769 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2 - 1..109179 109179


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T115326 WP_001044770.1 NZ_CP034125:c62765-62349 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T115326 NZ_CP034125:c62765-62349 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT115326 WP_001261282.1 NZ_CP034125:c62992-62762 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT115326 NZ_CP034125:c62992-62762 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References