Detailed information of TA system TA115321

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 124172..124842
• Replicon: plasmid p1b1
• Accession: NZ_CP034124
• Organism: Klebsiella pneumoniae strain BJCFK909

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: EH202_RS28375
• Protein ID: WP_004213072.1
• Coordinates: 124172..124615 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: EH202_RS28380
• Protein ID: WP_004213073.1
• Coordinates: 124612..124842 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH202_RS28325	119584..119859	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
EH202_RS28330	119922..120413	+	492	WP_011251327.1	DM13 domain-containing protein	-
EH202_RS28335	120462..121382	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
EH202_RS28345	121473..121879	+	407	Protein_135	GAF domain-containing protein	-
EH202_RS31450	122393..123028	-	636	Protein_136	response regulator	-
EH202_RS28365	123445..123738	+	294	Protein_137	transposase	-
EH202_RS28370	123742..124158	+	417	Protein_138	restriction endonuclease	-
EH202_RS28375	124172..124615	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
EH202_RS28380	124612..124842	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EH202_RS28385	125450..126583	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
EH202_RS28390	126599..126892	+	294	WP_004213076.1	hypothetical protein	-
EH202_RS28395	126882..127088	-	207	WP_004213077.1	hypothetical protein	-
EH202_RS28400	127440..127730	+	291	WP_004213078.1	hypothetical protein	-
EH202_RS28405	127720..128619	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..200216	200216

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T115321 WP_004213072.1 NZ_CP034124:c124615-124172 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T115321 NZ_CP034124:c124615-124172 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT115321 WP_004213073.1 NZ_CP034124:c124842-124612 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT115321 NZ_CP034124:c124842-124612 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620