Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 84093..85003 Replicon plasmid p1b1
Accession NZ_CP034124
Organism Klebsiella pneumoniae strain BJCFK909

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag EH202_RS28115 Protein ID WP_004026354.1
Coordinates 84093..84563 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag EH202_RS28120 Protein ID WP_004026357.1
Coordinates 84560..85003 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EH202_RS28070 79409..79723 + 315 WP_011251273.1 hypothetical protein -
EH202_RS28075 79732..80242 + 511 Protein_84 hypothetical protein -
EH202_RS28080 80232..80672 + 441 WP_011251275.1 hypothetical protein -
EH202_RS28090 81097..82053 - 957 WP_011251280.1 DsbA family protein -
EH202_RS28095 82113..82454 - 342 WP_011251281.1 hypothetical protein -
EH202_RS28100 82468..82779 - 312 WP_011251282.1 hypothetical protein -
EH202_RS28105 82796..83230 - 435 Protein_89 thioredoxin fold domain-containing protein -
EH202_RS28110 83247..83944 - 698 Protein_90 IS1 family transposase -
EH202_RS28115 84093..84563 - 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
EH202_RS28120 84560..85003 - 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
EH202_RS28125 85104..85607 - 504 Protein_93 DUF4113 domain-containing protein -
EH202_RS28130 85813..86793 - 981 WP_000019473.1 IS5-like element ISKpn26 family transposase -
EH202_RS28135 86859..88166 + 1308 Protein_95 FepA family TonB-dependent siderophore receptor -
EH202_RS28140 88228..88449 + 222 WP_004213615.1 hypothetical protein -
EH202_RS28145 88592..89494 - 903 WP_004213613.1 DMT family transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 1..200216 200216
- inside IScluster/Tn - iroN / rmpA 77812..97789 19977


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T115320 WP_004026354.1 NZ_CP034124:c84563-84093 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T115320 NZ_CP034124:c84563-84093 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT115320 WP_004026357.1 NZ_CP034124:c85003-84560 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT115320 NZ_CP034124:c85003-84560 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References