Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5392451..5393076 Replicon chromosome
Accession NZ_CP034123
Organism Klebsiella pneumoniae strain BJCFK909

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag EH202_RS27240 Protein ID WP_002882817.1
Coordinates 5392451..5392834 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag EH202_RS27245 Protein ID WP_004150355.1
Coordinates 5392834..5393076 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EH202_RS27225 5389817..5390719 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
EH202_RS27230 5390716..5391351 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
EH202_RS27235 5391348..5392277 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
EH202_RS27240 5392451..5392834 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
EH202_RS27245 5392834..5393076 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
EH202_RS27250 5393281..5394198 + 918 WP_002882812.1 alpha/beta hydrolase -
EH202_RS27255 5394212..5395153 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
EH202_RS27260 5395198..5395635 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
EH202_RS27265 5395632..5396492 - 861 WP_002882807.1 virulence factor BrkB family protein -
EH202_RS27270 5396486..5397085 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T115317 WP_002882817.1 NZ_CP034123:c5392834-5392451 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T115317 NZ_CP034123:c5392834-5392451 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT115317 WP_004150355.1 NZ_CP034123:c5393076-5392834 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT115317 NZ_CP034123:c5393076-5392834 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References