Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4194896..4195515 Replicon chromosome
Accession NZ_CP034123
Organism Klebsiella pneumoniae strain BJCFK909

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag EH202_RS21315 Protein ID WP_002892050.1
Coordinates 4195297..4195515 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag EH202_RS21310 Protein ID WP_002892066.1
Coordinates 4194896..4195270 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EH202_RS21300 4190048..4191241 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
EH202_RS21305 4191264..4194410 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit -
EH202_RS21310 4194896..4195270 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
EH202_RS21315 4195297..4195515 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
EH202_RS21320 4195674..4196240 + 567 WP_002892030.1 maltose O-acetyltransferase -
EH202_RS31195 4196212..4196352 - 141 WP_004147370.1 hypothetical protein -
EH202_RS21325 4196373..4196843 + 471 WP_002892026.1 YlaC family protein -
EH202_RS21330 4196818..4198269 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
EH202_RS21335 4198370..4199068 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
EH202_RS21340 4199065..4199205 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
EH202_RS21345 4199205..4199468 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T115313 WP_002892050.1 NZ_CP034123:4195297-4195515 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T115313 NZ_CP034123:4195297-4195515 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT115313 WP_002892066.1 NZ_CP034123:4194896-4195270 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT115313 NZ_CP034123:4194896-4195270 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References