Detailed information of TA system TA115292

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 1690107..1690909
• Replicon: chromosome
• Accession: NZ_CP034115
• Organism: Staphylococcus epidermidis strain CDC121

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: Q5HN83
• Locus tag: EH163_RS08650
• Protein ID: WP_002468490.1
• Coordinates: 1690730..1690909 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: Q5HN82
• Locus tag: EH163_RS08645
• Protein ID: WP_002456349.1
• Coordinates: 1690107..1690706 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH163_RS08625	1685319..1686776	+	1458	WP_002440300.1	ABC transporter substrate-binding protein/permease	-
EH163_RS08630	1686769..1687491	+	723	WP_001829838.1	amino acid ABC transporter ATP-binding protein	-
EH163_RS12960	1687988..1688137	+	150	WP_002468809.1	hypothetical protein	-
EH163_RS08635	1688352..1689482	+	1131	WP_001829814.1	tRNA epoxyqueuosine(34) reductase QueG	-
EH163_RS08640	1689479..1689949	+	471	WP_001829836.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
EH163_RS08645	1690107..1690706	+	600	WP_002456349.1	glucosamine-6-phosphate isomerase	Antitoxin
EH163_RS08650	1690730..1690909	+	180	WP_002468490.1	hypothetical protein	Toxin
EH163_RS08655	1691065..1691469	+	405	WP_001829818.1	hypothetical protein	-
EH163_RS08660	1691654..1693039	+	1386	WP_001829862.1	class II fumarate hydratase	-
EH163_RS08665	1693400..1694089	-	690	Protein_1577	RluA family pseudouridine synthase	-
EH163_RS08670	1694212..1695390	-	1179	WP_104832450.1	IS110 family transposase	-
EH163_RS08675	1695662..1695784	-	123	Protein_1579	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1694212..1695285	1073

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (130-195)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6963.56 Da
• Isoelectric Point: 4.3016

>T115292 WP_002468490.1 NZ_CP034115:1690730-1690909 [Staphylococcus epidermidis]
MANKKDSKLNYHEEENAMVTDLDDLKELGKEMEQISQENDEEKLNQSHDNEVRSDLKKQ

Nucleotide

• Length: 180 bp

>T115292 NZ_CP034115:1690730-1690909 [Staphylococcus epidermidis]
ATGGCGAACAAAAAAGATTCCAAATTAAATTATCATGAAGAAGAAAATGCTATGGTTACCGACTTAGATGATTTAAAAGA
ATTAGGAAAAGAAATGGAACAGATTTCACAGGAAAATGATGAAGAAAAATTAAATCAATCTCATGATAACGAAGTTCGTT
CAGATTTAAAAAAACAGTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22709.62 Da
• Isoelectric Point: 4.9942

>AT115292 WP_002456349.1 NZ_CP034115:1690107-1690706 [Staphylococcus epidermidis]
MAMNFKVFDNSEKVAEYTADILRKQFNNNPTTIAGFHLSKEHAPVFDELKKNVENHTVDFSQINILDYDDNHSYYEALGV
PTGQIYSISYENDAIDFISDKIKTKENKGKLTMQVLTIDENGKLDISVRQGLMEAREIFLVITGANKRDMVEKLYRENGK
TSFEPSDLKAHRMVNVILDKEAAAGLPEDVKEYFTARFA

Nucleotide

• Length: 600 bp

>AT115292 NZ_CP034115:1690107-1690706 [Staphylococcus epidermidis]
ATGGCAATGAATTTTAAAGTATTTGATAATAGTGAAAAAGTAGCAGAATATACTGCAGATATTCTACGTAAGCAATTTAA
TAATAATCCAACTACTATTGCAGGATTTCATTTGAGCAAAGAACATGCACCAGTGTTTGATGAACTTAAGAAAAATGTAG
AAAATCATACAGTTGATTTCAGTCAAATAAATATTTTAGATTATGATGATAATCATTCTTATTATGAGGCTTTGGGTGTG
CCAACAGGCCAAATTTATTCCATTTCTTACGAAAATGATGCAATTGACTTTATCTCAGATAAGATTAAGACTAAAGAAAA
TAAAGGCAAACTGACAATGCAAGTCCTTACGATTGATGAAAATGGTAAACTTGACATTAGCGTTCGTCAAGGTTTGATGG
AAGCTAGAGAAATTTTCTTAGTTATTACTGGTGCTAATAAACGCGATATGGTAGAAAAACTTTACCGTGAAAATGGTAAG
ACTAGTTTTGAACCATCGGATTTAAAAGCACACCGTATGGTCAATGTTATCTTAGATAAAGAAGCGGCTGCAGGCTTACC
TGAAGACGTAAAAGAGTACTTCACTGCACGTTTTGCATAA

Structures

Toxin

• AlphaFold DB: A0A2G7HY44

Antitoxin

• AlphaFold DB: A0A5E9LUD0