Detailed information of TA system TA115279

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 8750..9243
• Replicon: plasmid pRCAD0514EC-3
• Accession: NZ_CP034109
• Organism: Escherichia coli strain RCAD0514

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9Y9W8
• Locus tag: EGA47_RS23870
• Protein ID: WP_001230709.1
• Coordinates: 8989..9243 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: U9Y4R8
• Locus tag: EGA47_RS23865
• Protein ID: WP_000151456.1
• Coordinates: 8750..8992 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGA47_RS23820	3978..4670	+	693	WP_205962687.1	hypothetical protein	-
EGA47_RS23825	4706..5146	+	441	WP_023223352.1	hypothetical protein	-
EGA47_RS23830	5148..5474	+	327	WP_000206697.1	hypothetical protein	-
EGA47_RS23835	5734..6033	-	300	WP_034169410.1	type II toxin-antitoxin system HicB family antitoxin	-
EGA47_RS23840	6535..6717	+	183	WP_001428470.1	hypothetical protein	-
EGA47_RS23845	6805..7152	-	348	WP_000681609.1	type II toxin-antitoxin system HicB family antitoxin	-
EGA47_RS23850	7149..7397	-	249	WP_000513532.1	type II toxin-antitoxin system HicA family toxin	-
EGA47_RS23855	7886..8179	-	294	WP_000673428.1	hypothetical protein	-
EGA47_RS23860	8311..8544	-	234	WP_000553847.1	hypothetical protein	-
EGA47_RS23865	8750..8992	-	243	WP_000151456.1	hypothetical protein	Antitoxin
EGA47_RS23870	8989..9243	-	255	WP_001230709.1	hypothetical protein	Toxin
EGA47_RS23875	9240..9782	-	543	WP_001083904.1	hypothetical protein	-
EGA47_RS23880	9886..10401	-	516	WP_001025393.1	J domain-containing protein	-
EGA47_RS23885	11248..11497	-	250	Protein_17	hypothetical protein	-
EGA47_RS23890	11721..12386	+	666	WP_000532149.1	P-loop NTPase	-
EGA47_RS23895	12460..12744	+	285	WP_001162371.1	hypothetical protein	-
EGA47_RS23900	12762..13358	-	597	WP_000125165.1	hypothetical protein	-
EGA47_RS23905	13508..13669	-	162	WP_001366886.1	type I toxin-antitoxin system Hok family toxin	-
EGA47_RS23910	14053..14214	-	162	WP_001128311.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..60954	60954

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (25-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-69)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9942.47 Da
• Isoelectric Point: 10.1206

>T115279 WP_001230709.1 NZ_CP034109:c9243-8989 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Nucleotide

• Length: 255 bp

>T115279 NZ_CP034109:c9243-8989 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9136.45 Da
• Isoelectric Point: 7.2775

>AT115279 WP_000151456.1 NZ_CP034109:c8992-8750 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Nucleotide

• Length: 243 bp

>AT115279 NZ_CP034109:c8992-8750 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Structures

Toxin

• AlphaFold DB: A0A3G9H8F0

Antitoxin

• AlphaFold DB: A0A3G9GNF5