Detailed information of TA system TA115278

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 6805..7397
• Replicon: plasmid pRCAD0514EC-3
• Accession: NZ_CP034109
• Organism: Escherichia coli strain RCAD0514

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1I9ZJC5
• Locus tag: EGA47_RS23850
• Protein ID: WP_000513532.1
• Coordinates: 7149..7397 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1I9ZJ49
• Locus tag: EGA47_RS23845
• Protein ID: WP_000681609.1
• Coordinates: 6805..7152 (-)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGA47_RS23805	2092..2355	-	264	WP_148119646.1	plasmid mobilization relaxosome protein MobC	-
EGA47_RS23810	2804..3206	+	403	Protein_2	zinc transporter	-
EGA47_RS23815	3224..3976	+	753	WP_000797966.1	conjugal transfer protein TraL	-
EGA47_RS23820	3978..4670	+	693	WP_205962687.1	hypothetical protein	-
EGA47_RS23825	4706..5146	+	441	WP_023223352.1	hypothetical protein	-
EGA47_RS23830	5148..5474	+	327	WP_000206697.1	hypothetical protein	-
EGA47_RS23835	5734..6033	-	300	WP_034169410.1	type II toxin-antitoxin system HicB family antitoxin	-
EGA47_RS23840	6535..6717	+	183	WP_001428470.1	hypothetical protein	-
EGA47_RS23845	6805..7152	-	348	WP_000681609.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EGA47_RS23850	7149..7397	-	249	WP_000513532.1	type II toxin-antitoxin system HicA family toxin	Toxin
EGA47_RS23855	7886..8179	-	294	WP_000673428.1	hypothetical protein	-
EGA47_RS23860	8311..8544	-	234	WP_000553847.1	hypothetical protein	-
EGA47_RS23865	8750..8992	-	243	WP_000151456.1	hypothetical protein	-
EGA47_RS23870	8989..9243	-	255	WP_001230709.1	hypothetical protein	-
EGA47_RS23875	9240..9782	-	543	WP_001083904.1	hypothetical protein	-
EGA47_RS23880	9886..10401	-	516	WP_001025393.1	J domain-containing protein	-
EGA47_RS23885	11248..11497	-	250	Protein_17	hypothetical protein	-
EGA47_RS23890	11721..12386	+	666	WP_000532149.1	P-loop NTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..60954	60954

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9378.03 Da
• Isoelectric Point: 10.4800

>T115278 WP_000513532.1 NZ_CP034109:c7397-7149 [Escherichia coli]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Nucleotide

• Length: 249 bp

>T115278 NZ_CP034109:c7397-7149 [Escherichia coli]
ATGGGGAAAGCAGATAAGCTACTGGCAAAATTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTCGTTGTTTTGTT
TTCTTCTCTAGGATATGTCAAAAAGGAAATGCAGGGATCAAGAGTGCGATTTTTCAATGCTGAAATTAATCACACAATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAGCTATTAAACAGAACTTGAAAGAGGCTGGG
GTTTTATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13206.97 Da
• Isoelectric Point: 4.8313

>AT115278 WP_000681609.1 NZ_CP034109:c7152-6805 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA

Nucleotide

• Length: 348 bp

>AT115278 NZ_CP034109:c7152-6805 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGTACGGTTGAGCCGGATTTTGAAAATAATATCCTGTATGGAAAGCTGGC
ATTTATTCGTGATCTGGTGACTTATGAGGCAGAAACATTGGCTGACCTGGAACGGGAATTTAAAACATCGGTTGATTTGT
ATTTACAGTCCTGTGTGGAGGACGGAAAGGAGCCTGACACGCCCTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAG
CTGCATCGTCGGGTCGCTGAAATGGCGATGGAAGAAGATTTGTCGCTGAATGCCTTTGTCAATAAGGCACTGGAAAAAGA
AGTCAGTCATCATCGCGCAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A1I9ZJC5

Antitoxin

• AlphaFold DB: A0A1I9ZJ49