Detailed information of TA system TA115267

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 4477113..4477820
• Replicon: chromosome
• Accession: NZ_CP034106
• Organism: Escherichia coli strain RCAD0514

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A377AQ31
• Locus tag: EGA47_RS21595
• Protein ID: WP_032161355.1
• Coordinates: 4477113..4477454 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: EGA47_RS21600
• Protein ID: WP_032161354.1
• Coordinates: 4477485..4477820 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGA47_RS21565	4473092..4473742	-	651	WP_001037977.1	HNH endonuclease	-
EGA47_RS21570	4473879..4474022	+	144	Protein_4203	HNH endonuclease	-
EGA47_RS21575	4474656..4475444	-	789	WP_032161357.1	helix-turn-helix domain-containing protein	-
EGA47_RS21580	4475561..4475836	-	276	WP_001683517.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EGA47_RS21585	4475840..4476088	-	249	WP_024187601.1	ribbon-helix-helix domain-containing protein	-
EGA47_RS21590	4476165..4476998	-	834	WP_032250394.1	DUF4942 domain-containing protein	-
EGA47_RS21595	4477113..4477454	-	342	WP_032161355.1	TA system toxin CbtA family protein	Toxin
EGA47_RS21600	4477485..4477820	-	336	WP_032161354.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EGA47_RS21605	4477820..4478293	-	474	WP_032161353.1	DNA repair protein RadC	-
EGA47_RS21610	4478323..4479141	-	819	WP_001683514.1	DUF945 domain-containing protein	-
EGA47_RS21615	4479377..4479841	-	465	Protein_4212	hypothetical protein	-
EGA47_RS21620	4479858..4480555	-	698	Protein_4213	IS1 family transposase	-
EGA47_RS21625	4480649..4481101	+	453	WP_152925145.1	hypothetical protein	-
EGA47_RS21630	4481325..4482305	-	981	WP_000019450.1	IS5-like element ISKpn26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimE / fimB	4467065..4482923	15858

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-110)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13048.01 Da
• Isoelectric Point: 9.8953

>T115267 WP_032161355.1 NZ_CP034106:c4477454-4477113 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQTLLTRLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVDAVNFLVEKYELIRIDRKGF
SWQEQTPYLRAVDILRARQATGLLRRSHNTTAR

Nucleotide

• Length: 342 bp

>T115267 NZ_CP034106:c4477454-4477113 [Escherichia coli]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCGCCCGTCGTGGTCTGGCAAACATTACTGAC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTCGATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGATCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCAGTCGATATTTTGCGGGCGCGGCAGGCTACAGGTCTGCTGCGTCGCAG
CCATAACACCACGGCACGGTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12530.56 Da
• Isoelectric Point: 8.1281

>AT115267 WP_032161354.1 NZ_CP034106:c4477820-4477485 [Escherichia coli]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSACQKR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT115267 NZ_CP034106:c4477820-4477485 [Escherichia coli]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGCTGATGAAACAACTGGAGCTGAAACTGGCTGACGGTGAACTCTCCGCCTGCCAAAAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Structures

Toxin

• AlphaFold DB: A0A377AQ31