Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4132703..4133490 Replicon chromosome
Accession NZ_CP034106
Organism Escherichia coli strain RCAD0514

Toxin (Protein)


Gene name yeeV Uniprot ID A0A377E3D6
Locus tag EGA47_RS19955 Protein ID WP_001194695.1
Coordinates 4133113..4133490 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A2H9G3E7
Locus tag EGA47_RS19950 Protein ID WP_000066236.1
Coordinates 4132703..4133062 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGA47_RS19905 4127943..4128395 + 453 WP_001020418.1 hypothetical protein -
EGA47_RS19910 4128392..4128844 + 453 WP_000734138.1 hypothetical protein -
EGA47_RS19915 4128907..4129449 + 543 WP_001104018.1 DUF4339 domain-containing protein -
EGA47_RS19920 4129509..4129961 + 453 WP_001061894.1 hypothetical protein -
EGA47_RS19925 4130038..4130271 + 234 WP_001114682.1 DUF905 domain-containing protein -
EGA47_RS19930 4130391..4131209 + 819 WP_124846291.1 DUF945 domain-containing protein -
EGA47_RS19935 4131543..4131946 + 404 Protein_3886 antirestriction protein -
EGA47_RS19940 4131958..4132437 + 480 WP_000437750.1 DNA repair protein RadC -
EGA47_RS19945 4132458..4132679 + 222 WP_000691981.1 DUF987 domain-containing protein -
EGA47_RS19950 4132703..4133062 + 360 WP_000066236.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
EGA47_RS19955 4133113..4133490 + 378 WP_001194695.1 TA system toxin CbtA family protein Toxin
EGA47_RS19960 4133487..4133978 + 492 WP_000777682.1 hypothetical protein -
EGA47_RS19965 4134010..4134213 + 204 WP_000413747.1 DUF957 domain-containing protein -
EGA47_RS19970 4134294..4135138 + 845 Protein_3893 DUF4942 domain-containing protein -
EGA47_RS19980 4135480..4136211 - 732 WP_001340895.1 DNA polymerase III subunit epsilon -
EGA47_RS19985 4136276..4136743 + 468 WP_000917883.1 ribonuclease HI -
EGA47_RS19990 4136740..4137462 - 723 WP_001326702.1 class I SAM-dependent methyltransferase -
EGA47_RS19995 4137496..4138251 + 756 WP_001052715.1 hydroxyacylglutathione hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(15-114)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14180.97 Da        Isoelectric Point: 7.4209

>T115266 WP_001194695.1 NZ_CP034106:4133113-4133490 [Escherichia coli]
MQTQPLSSTQEATSRPSPVEIWQRLLSHLLDRHYGLTLNDTPFGNDGVIQEHIDAGISLCDAVNFIVEKYDLVRIDRHGF
STETQLPRLTSIDILRARKATGLMTRNDYRTVTDITTGKYRGGHR

Download         Length: 378 bp

>T115266 NZ_CP034106:4133113-4133490 [Escherichia coli]
ATGCAAACCCAACCACTATCCTCAACGCAGGAGGCTACATCACGCCCGTCACCGGTGGAGATCTGGCAACGGCTTCTGAG
CCACCTGTTGGATCGTCACTACGGTCTGACGCTTAACGACACGCCGTTTGGCAACGACGGTGTGATTCAGGAGCATATCG
ACGCCGGTATATCACTGTGCGACGCCGTGAATTTTATTGTGGAGAAGTACGATCTGGTGCGCATCGATCGTCACGGTTTC
AGTACAGAAACACAGTTGCCGCGTCTCACCAGTATCGATATTCTCCGCGCCCGCAAAGCCACCGGGTTGATGACTCGCAA
CGATTACAGAACGGTGACCGACATCACCACCGGTAAATACCGTGGGGGGCATCGATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13127.02 Da        Isoelectric Point: 7.0261

>AT115266 WP_000066236.1 NZ_CP034106:4132703-4133062 [Escherichia coli]
MSNKIPTVNHDITEPWWGLKRSITPCFGARLVQSGNCLHYLADRASITGQFSDGDLRHLDQAFPLLLKQLELMLTSGELT
PRHQHCVTLYAKGLTCEADSLGSHGYVYLAIYPTPAATA

Download         Length: 360 bp

>AT115266 NZ_CP034106:4132703-4133062 [Escherichia coli]
TTGTCGAATAAAATACCGACAGTAAACCACGATATCACTGAACCCTGGTGGGGGCTTAAACGCAGTATCACGCCCTGTTT
TGGCGCACGGCTGGTGCAGTCGGGCAACTGTCTGCATTATCTCGCTGACCGGGCCAGCATCACCGGACAGTTCAGCGACG
GGGATTTACGGCATCTCGACCAGGCATTTCCACTGCTGCTCAAACAGCTTGAACTGATGCTCACCAGCGGTGAACTCACT
CCCCGCCATCAGCACTGTGTCACGCTCTATGCAAAAGGGCTGACCTGCGAGGCCGACTCCCTCGGCTCGCATGGTTACGT
ATACCTGGCGATTTATCCGACACCAGCGGCAACCGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A377E3D6

Antitoxin

Source ID Structure
AlphaFold DB A0A2H9G3E7

References