Detailed information of TA system TA115262

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2900892..2901530
• Replicon: chromosome
• Accession: NZ_CP034106
• Organism: Escherichia coli strain RCAD0514

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: EGA47_RS13930
• Protein ID: WP_000813794.1
• Coordinates: 2901354..2901530 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: EGA47_RS13925
• Protein ID: WP_105966292.1
• Coordinates: 2900892..2901308 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGA47_RS13905	2896044..2896985	-	942	WP_001251319.1	ABC transporter permease	-
EGA47_RS13910	2896986..2897999	-	1014	WP_000220393.1	ABC transporter ATP-binding protein	-
EGA47_RS13915	2898017..2899162	-	1146	WP_124846286.1	ABC transporter substrate-binding protein	-
EGA47_RS13920	2899407..2900813	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
EGA47_RS13925	2900892..2901308	-	417	WP_105966292.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
EGA47_RS13930	2901354..2901530	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
EGA47_RS13935	2901752..2901982	+	231	WP_000494244.1	YncJ family protein	-
EGA47_RS13940	2902074..2904035	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
EGA47_RS13945	2904108..2904644	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
EGA47_RS13950	2904697..2905911	+	1215	WP_072003393.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-67)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T115262 WP_000813794.1 NZ_CP034106:c2901530-2901354 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T115262 NZ_CP034106:c2901530-2901354 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15228.60 Da
• Isoelectric Point: 4.7386

>AT115262 WP_105966292.1 NZ_CP034106:c2901308-2900892 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVIV

Nucleotide

• Length: 417 bp

>AT115262 NZ_CP034106:c2901308-2900892 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATAGTTTAA

Structures

Toxin

• PDB: 6HPB