Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1254359..1255013 Replicon chromosome
Accession NZ_CP034106
Organism Escherichia coli strain RCAD0514

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag EGA47_RS05955 Protein ID WP_000244777.1
Coordinates 1254606..1255013 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EGA47_RS05950 Protein ID WP_000354046.1
Coordinates 1254359..1254625 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGA47_RS05925 1249528..1250271 + 744 WP_000951962.1 SDR family oxidoreductase -
EGA47_RS05930 1250328..1251761 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
EGA47_RS05935 1251806..1252117 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
EGA47_RS05940 1252281..1252940 + 660 WP_000250274.1 hemolysin III family protein -
EGA47_RS05945 1253136..1254116 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
EGA47_RS05950 1254359..1254625 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EGA47_RS05955 1254606..1255013 + 408 WP_000244777.1 toxin CptA Toxin
EGA47_RS05960 1255053..1255574 - 522 WP_001055874.1 flavodoxin FldB -
EGA47_RS05965 1255686..1256582 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EGA47_RS05970 1256607..1257317 + 711 WP_124846177.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EGA47_RS05975 1257323..1259056 + 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T115249 WP_000244777.1 NZ_CP034106:1254606-1255013 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T115249 NZ_CP034106:1254606-1255013 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT115249 WP_000354046.1 NZ_CP034106:1254359-1254625 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT115249 NZ_CP034106:1254359-1254625 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References