Detailed information of TA system TA115240

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/GNAT-DUF1778
• Location: 2510693..2511438
• Replicon: chromosome
• Accession: NZ_CP034105
• Organism: Proteus sp. GOKU

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: EHE21_RS11715
• Protein ID: WP_194950769.1
• Coordinates: 2510947..2511438 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: EHE21_RS11710
• Protein ID: WP_075673024.1
• Coordinates: 2510693..2510959 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHE21_RS11675	2506354..2506836	+	483	WP_006533151.1	flagellar basal body-associated protein FliL	-
EHE21_RS11680	2506842..2507873	+	1032	WP_023581831.1	flagellar motor switch protein FliM	-
EHE21_RS11685	2507866..2508276	+	411	WP_023581830.1	flagellar motor switch protein FliN	-
EHE21_RS11690	2508280..2508726	+	447	WP_023581829.1	flagellar biosynthetic protein FliO	-
EHE21_RS11695	2508783..2509496	+	714	WP_171455369.1	flagellar type III secretion system pore protein FliP	-
EHE21_RS11700	2509512..2509781	+	270	WP_069367481.1	flagellar biosynthesis protein FliQ	-
EHE21_RS11705	2509787..2510569	+	783	WP_088495227.1	flagellar type III secretion system protein FliR	-
EHE21_RS11710	2510693..2510959	+	267	WP_075673024.1	DUF1778 domain-containing protein	Antitoxin
EHE21_RS11715	2510947..2511438	+	492	WP_194950769.1	GNAT family N-acetyltransferase	Toxin
EHE21_RS11720	2511523..2512467	-	945	WP_194950770.1	flagellar hook-associated protein FlgL	-
EHE21_RS11725	2512499..2514142	-	1644	WP_194950771.1	flagellar hook-associated protein FlgK	-
EHE21_RS11730	2514260..2515246	-	987	WP_194950772.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
EHE21_RS11735	2515246..2516352	-	1107	WP_194950773.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (22-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 18225.13 Da
• Isoelectric Point: 10.3494

>T115240 WP_194950769.1 NZ_CP034105:2510947-2511438 [Proteus sp. GOKU]
MGKVTAPEPLSNSHEVADFYSSENVLDNWIKQRGLKNQFLGASRTFVVCEKNKKRVVGYYSLATGSVNHTEAISHIRRNM
PDPIPVIILARLAVDKTFHGRGLGADLLRDAVLRCHHVAENIGVRAIMVHALTESAKHFYLHHGFKASSTQEKILFLALK
KKI

Nucleotide

• Length: 492 bp

>T115240 NZ_CP034105:2510947-2511438 [Proteus sp. GOKU]
ATGGGAAAAGTAACAGCACCTGAGCCGTTATCAAATTCACATGAAGTGGCTGATTTTTATAGTAGTGAAAATGTATTAGA
TAACTGGATAAAACAAAGAGGTTTAAAAAACCAATTCTTAGGTGCATCACGTACATTTGTGGTCTGTGAAAAAAATAAAA
AACGTGTTGTTGGGTACTATTCTTTAGCGACAGGTAGTGTTAACCATACTGAAGCAATTAGTCATATTCGTCGTAATATG
CCAGATCCAATACCCGTGATCATTTTAGCTAGACTGGCTGTTGATAAAACTTTTCATGGAAGAGGCCTTGGTGCCGATTT
GTTAAGAGATGCTGTTTTGCGTTGTCATCATGTAGCTGAAAACATTGGTGTTAGAGCGATTATGGTTCATGCTTTAACTG
AAAGCGCAAAACATTTTTATCTTCATCATGGTTTCAAAGCTTCTTCGACTCAAGAAAAAATCTTATTCTTGGCATTAAAA
AAGAAAATATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10111.62 Da
• Isoelectric Point: 4.5256

>AT115240 WP_075673024.1 NZ_CP034105:2510693-2510959 [Proteus sp. GOKU]
MKADVQLNIRAKESQRALIDTAAEILHKSRTDFILETACQAAEDVILDRRTFNLNDAQYAEFIEIIDAPVDIDPALEKLL
MRKPLWEK

Nucleotide

• Length: 267 bp

>AT115240 NZ_CP034105:2510693-2510959 [Proteus sp. GOKU]
ATGAAAGCTGACGTACAACTCAATATTCGCGCTAAAGAATCACAACGCGCACTTATTGATACTGCTGCTGAAATTCTCCA
TAAATCTCGTACTGACTTTATTCTTGAGACAGCGTGCCAAGCTGCTGAAGACGTCATTTTAGACCGTAGAACCTTTAATT
TAAATGATGCTCAATATGCGGAATTTATAGAAATTATTGATGCGCCGGTTGATATTGACCCTGCTCTTGAAAAGTTATTA
ATGAGAAAACCATTATGGGAAAAGTAA