115236

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 960995..961554 Replicon chromosome
Accession NZ_CP034103
Organism Brucella melitensis strain BmWS93

Toxin (Protein)


Gene name brnT Uniprot ID A0A7U8PD14
Locus tag EHE09_RS05040 Protein ID WP_002966807.1
Coordinates 961309..961554 (-) Length 82 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID C0RIV1
Locus tag EHE09_RS05035 Protein ID WP_004683740.1
Coordinates 960995..961309 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EHE09_RS04985 956700..956903 - 204 WP_004683737.1 hypothetical protein -
EHE09_RS04990 956906..957121 - 216 WP_005968947.1 hypothetical protein -
EHE09_RS04995 957118..957321 - 204 WP_002964088.1 hypothetical protein -
EHE09_RS05000 957371..958075 + 705 WP_005968944.1 hypothetical protein -
EHE09_RS05005 958072..958302 + 231 WP_002964091.1 hypothetical protein -
EHE09_RS05010 958299..958574 + 276 WP_002964092.1 hypothetical protein -
EHE09_RS05015 958597..959073 + 477 WP_002966806.1 hypothetical protein -
EHE09_RS05020 959419..960129 + 711 WP_004683739.1 porin family protein -
EHE09_RS05025 960477..960647 - 171 WP_002964095.1 DUF1508 domain-containing protein -
EHE09_RS05035 960995..961309 - 315 WP_004683740.1 BrnA antitoxin family protein Antitoxin
EHE09_RS05040 961309..961554 - 246 WP_002966807.1 BrnT family toxin Toxin
EHE09_RS05045 961687..962325 + 639 Protein_923 tRNA-guanine transglycosylase -
EHE09_RS05050 962395..963153 + 759 Protein_924 IS5 family transposase -
EHE09_RS05055 963154..963846 + 693 WP_006135935.1 hypothetical protein -
EHE09_RS05060 964258..965709 + 1452 WP_006135932.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - wboA / wbdA 954928..969025 14097
- flank IS/Tn - - 962395..962763 368


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-78)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-102)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 9162.82 Da        Isoelectric Point: 10.3158

>T115236 WP_002966807.1 NZ_CP034103:c961554-961309 [Brucella melitensis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Download         Length: 246 bp

>T115236 NZ_CP034103:c961554-961309 [Brucella melitensis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11653.48 Da        Isoelectric Point: 10.5824

>AT115236 WP_004683740.1 NZ_CP034103:c961309-960995 [Brucella melitensis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Download         Length: 315 bp

>AT115236 NZ_CP034103:c961309-960995 [Brucella melitensis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U8PD14

Antitoxin

Source ID Structure
AlphaFold DB C0RIV1

References