Detailed information of TA system TA115228

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2187580..2188109
• Replicon: chromosome
• Accession: NZ_CP034102
• Organism: Staphylococcus aureus strain O267

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SaO267_RS11550
• Protein ID: WP_000621175.1
• Coordinates: 2187580..2187942 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SaO267_RS11555
• Protein ID: WP_000948331.1
• Coordinates: 2187939..2188109 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SaO267_RS11525	2184558..2185328	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
SaO267_RS11530	2185303..2185782	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
SaO267_RS11535	2185784..2186110	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SaO267_RS11540	2186229..2187230	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SaO267_RS11550	2187580..2187942	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SaO267_RS11555	2187939..2188109	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SaO267_RS11560	2188194..2189342	-	1149	WP_001281150.1	alanine racemase	-
SaO267_RS11565	2189408..2189767	-	360	WP_000581198.1	holo-ACP synthase	-
SaO267_RS11570	2189771..2190262	-	492	WP_001286982.1	PH domain-containing protein	-
SaO267_RS11575	2190255..2191832	-	1578	WP_001294643.1	PH domain-containing protein	-
SaO267_RS11580	2191825..2192304	-	480	WP_001287084.1	hypothetical protein	-
SaO267_RS11585	2192513..2193073	-	561	WP_001092415.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T115228 WP_000621175.1 NZ_CP034102:c2187942-2187580 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T115228 NZ_CP034102:c2187942-2187580 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT115228 WP_000948331.1 NZ_CP034102:c2188109-2187939 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT115228 NZ_CP034102:c2188109-2187939 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1