Detailed information of TA system TA115199

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3115476..3116497
• Replicon: chromosome
• Accession: NZ_CP034091
• Organism: Proteus mirabilis strain PmBC1123

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: EHQ66_RS14610
• Protein ID: WP_107033620.1
• Coordinates: 3115928..3116497 (+)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: B4F2H3
• Locus tag: EHQ66_RS14605
• Protein ID: WP_004245528.1
• Coordinates: 3115476..3115928 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHQ66_RS14585	3111262..3111792	-	531	WP_004245524.1	RNA pyrophosphohydrolase	-
EHQ66_RS14590	3112061..3113074	+	1014	WP_107033621.1	thiamine ABC transporter substrate binding subunit	-
EHQ66_RS14595	3113074..3114654	+	1581	WP_124743813.1	thiamine/thiamine pyrophosphate ABC transporter permease ThiP	-
EHQ66_RS14600	3114647..3115348	+	702	WP_004245527.1	thiamine ABC transporter ATP-binding protein ThiQ	-
EHQ66_RS14605	3115476..3115928	+	453	WP_004245528.1	helix-turn-helix domain-containing protein	Antitoxin
EHQ66_RS14610	3115928..3116497	+	570	WP_107033620.1	PIN domain-containing protein	Toxin
EHQ66_RS14615	3116613..3118967	+	2355	WP_107033619.1	DNA polymerase II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• SinI-like, DNA-binding domain (80-125)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21627.17 Da
• Isoelectric Point: 7.0180

>T115199 WP_107033620.1 NZ_CP034091:3115928-3116497 [Proteus mirabilis]
MTNYTVLLDACVMYPAPLRSYLMYLANTGLFRARWSNQIHDEWITNLLVNNPTLTAERLIRTRDLMNAHVPDCLVEGYEP
LICGLKLPDPNDRHVLAAAIKGQAQAIITFNLKDFPQKYLNSFDILAIHPDEFLSDMFELNPSSCIKAAQQHRQSLKDPP
MTVETFLGCLQKQKLPQFISHLRAFQILL

Nucleotide

• Length: 570 bp

>T115199 NZ_CP034091:3115928-3116497 [Proteus mirabilis]
ATGACAAACTATACTGTTTTGCTTGATGCCTGTGTCATGTATCCAGCCCCTCTTAGAAGTTATTTAATGTATCTTGCTAA
TACTGGTTTATTTAGAGCACGTTGGAGTAATCAGATCCATGATGAATGGATCACTAATTTATTAGTCAACAATCCAACAT
TAACAGCTGAGCGACTAATACGAACCAGAGATTTAATGAATGCACATGTTCCTGATTGTTTAGTAGAAGGCTACGAACCG
CTTATTTGTGGTTTAAAGCTACCTGATCCTAATGATCGTCATGTGCTAGCAGCAGCAATAAAAGGACAGGCTCAAGCTAT
AATTACATTTAACTTAAAAGATTTTCCGCAAAAATACCTTAATTCTTTTGATATTTTAGCTATCCATCCTGATGAATTTT
TGAGTGACATGTTTGAATTAAATCCTAGTAGCTGTATTAAAGCGGCGCAACAACATAGACAAAGCCTAAAGGATCCACCA
ATGACCGTAGAGACCTTTTTAGGCTGTTTACAAAAGCAGAAATTACCCCAGTTTATTTCCCATTTACGGGCATTCCAGAT
ATTATTATAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16700.15 Da
• Isoelectric Point: 5.7723

>AT115199 WP_004245528.1 NZ_CP034091:3115476-3115928 [Proteus mirabilis]
MNTISERLSAEDIALAKLSSQELSAVMDINGKTQKLSIIDKTGNNHIVQIPNSALRLMIEILTQLGQGNTVSITPIHAEL
TTQEAADILNMSRPTFVKLLDTRAIPYSRKGNRRKVAYADIVAYKNELEQKRLDALAELSQLDQEMDMGY

Nucleotide

• Length: 453 bp

>AT115199 NZ_CP034091:3115476-3115928 [Proteus mirabilis]
ATGAATACGATAAGTGAGAGACTTAGTGCTGAAGATATTGCATTAGCAAAATTAAGTAGCCAAGAGCTATCCGCTGTTAT
GGATATTAATGGCAAAACGCAGAAGCTATCCATTATAGATAAGACGGGTAATAATCATATTGTACAAATACCAAATAGTG
CATTACGCCTAATGATTGAAATTCTTACTCAACTAGGGCAAGGGAATACTGTTAGTATCACACCAATCCATGCTGAGCTA
ACGACACAGGAAGCGGCTGATATACTCAATATGTCCCGTCCTACATTTGTAAAATTATTAGATACACGAGCGATACCCTA
CTCTAGAAAAGGAAATCGTAGGAAAGTTGCTTATGCTGATATTGTTGCTTATAAAAATGAATTGGAGCAAAAACGACTTG
ATGCCCTTGCTGAATTATCGCAATTAGATCAAGAAATGGATATGGGGTATTAA

Structures

Antitoxin

• AlphaFold DB: B4F2H3