Detailed information of TA system TA115194

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 78329..79350
• Replicon: chromosome
• Accession: NZ_CP034090
• Organism: Proteus mirabilis strain PmSC1111

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: EHQ78_RS00325
• Protein ID: WP_004249488.1
• Coordinates: 78329..78898 (-)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: B4F2H3
• Locus tag: EHQ78_RS00330
• Protein ID: WP_004245528.1
• Coordinates: 78898..79350 (-)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHQ78_RS00320	75859..78213	-	2355	WP_063108627.1	DNA polymerase II	-
EHQ78_RS00325	78329..78898	-	570	WP_004249488.1	PIN domain-containing protein	Toxin
EHQ78_RS00330	78898..79350	-	453	WP_004245528.1	helix-turn-helix domain-containing protein	Antitoxin
EHQ78_RS00335	79478..80179	-	702	WP_063108628.1	thiamine ABC transporter ATP-binding protein ThiQ	-
EHQ78_RS00340	80172..81752	-	1581	WP_046334723.1	thiamine/thiamine pyrophosphate ABC transporter permease ThiP	-
EHQ78_RS00345	81752..82765	-	1014	WP_114138075.1	thiamine ABC transporter substrate binding subunit	-
EHQ78_RS00350	83034..83564	+	531	WP_004245524.1	RNA pyrophosphohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• SinI-like, DNA-binding domain (80-125)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21600.10 Da
• Isoelectric Point: 7.3470

>T115194 WP_004249488.1 NZ_CP034090:c78898-78329 [Proteus mirabilis]
MTNYTVLLDACVMYPAPLRSYLMYLANTGLFRARWSNQIHDEWITNLLVNNPTLTAERLIRTRDLMNAHVPDCLVEGYEP
LICGLKLPDPNDRHVLAAAIKGQAQAIITFNLKDFPQKYLNSFDISAIHPDEFLSDMFELNPSSCIKAAQQHRQSLKNPP
MTVETFLGCLQKQKLPQFISHLRAFQILL

Nucleotide

• Length: 570 bp

>T115194 NZ_CP034090:c78898-78329 [Proteus mirabilis]
ATGACAAACTATACTGTTTTGCTTGATGCCTGTGTCATGTATCCAGCCCCTCTTAGAAGTTATTTAATGTATCTTGCTAA
TACTGGTTTATTTAGAGCACGTTGGAGTAATCAGATCCATGATGAATGGATCACTAATTTATTAGTCAACAATCCAACAT
TAACAGCTGAGCGACTAATACGAACCAGAGATTTAATGAATGCACATGTTCCTGATTGTTTAGTAGAAGGCTACGAACCG
CTTATTTGTGGTTTAAAGCTACCTGATCCTAATGATCGTCATGTGCTAGCAGCAGCAATAAAAGGACAGGCTCAAGCTAT
AATTACATTTAACTTAAAAGATTTTCCGCAAAAATACCTTAATTCTTTTGATATTTCAGCTATCCATCCTGATGAATTTT
TGAGTGACATGTTTGAATTAAATCCTAGTAGCTGTATTAAAGCGGCGCAACAACATAGACAAAGCCTAAAGAATCCACCA
ATGACCGTAGAGACCTTTTTAGGCTGTTTACAAAAGCAGAAATTACCCCAGTTTATTTCCCATTTACGGGCATTCCAGAT
ATTATTATAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16700.15 Da
• Isoelectric Point: 5.7723

>AT115194 WP_004245528.1 NZ_CP034090:c79350-78898 [Proteus mirabilis]
MNTISERLSAEDIALAKLSSQELSAVMDINGKTQKLSIIDKTGNNHIVQIPNSALRLMIEILTQLGQGNTVSITPIHAEL
TTQEAADILNMSRPTFVKLLDTRAIPYSRKGNRRKVAYADIVAYKNELEQKRLDALAELSQLDQEMDMGY

Nucleotide

• Length: 453 bp

>AT115194 NZ_CP034090:c79350-78898 [Proteus mirabilis]
ATGAATACGATAAGTGAGAGACTTAGTGCTGAAGATATTGCATTAGCAAAATTAAGTAGCCAAGAGCTATCCGCTGTTAT
GGATATTAATGGCAAAACGCAGAAGCTATCCATTATAGATAAGACGGGTAATAATCATATTGTACAAATACCAAATAGTG
CATTACGCCTAATGATTGAAATTCTTACTCAACTAGGGCAAGGGAATACTGTTAGTATCACACCAATCCATGCTGAGCTA
ACGACGCAGGAAGCGGCTGATATACTCAATATGTCCCGTCCTACATTTGTAAAATTATTAGATACACGAGCGATACCCTA
CTCTAGAAAAGGAAATCGTAGGAAAGTTGCTTATGCTGATATTGTTGCTTATAAAAATGAATTGGAGCAAAAACGACTTG
ATGCCCTTGCTGAATTATCGCAATTAGATCAAGAAATGGATATGGGGTATTAA

Structures

Antitoxin

• AlphaFold DB: B4F2H3