Detailed information of TA system TA115191

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 254331..255124
• Replicon: plasmid pGW6_1
• Accession: NZ_CP034087
• Organism: Methylocystis rosea strain GW6

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: -
• Locus tag: EHO51_RS18965
• Protein ID: WP_124740407.1
• Coordinates: 254603..255124 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: -
• Locus tag: EHO51_RS18960
• Protein ID: WP_124740406.1
• Coordinates: 254331..254606 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHO51_RS18930	249452..249886	-	435	WP_124740402.1	EamA family transporter	-
EHO51_RS18935	250263..251284	+	1022	Protein_222	tryptophan--tRNA ligase	-
EHO51_RS18940	251362..252200	+	839	WP_124740403.1	IS5 family transposase	-
EHO51_RS18945	252374..252988	-	615	WP_124740404.1	NYN domain-containing protein	-
EHO51_RS18950	253043..253980	-	938	Protein_225	tyrosine-type recombinase/integrase	-
EHO51_RS18955	253977..254195	-	219	WP_124740405.1	hypothetical protein	-
EHO51_RS18960	254331..254606	+	276	WP_124740406.1	DUF1778 domain-containing protein	Antitoxin
EHO51_RS18965	254603..255124	+	522	WP_124740407.1	GNAT family N-acetyltransferase	Toxin
EHO51_RS18970	255367..256794	-	1428	WP_124740408.1	IS1182 family transposase	-
EHO51_RS18975	256911..257520	-	610	Protein_230	DUF2269 domain-containing protein	-
EHO51_RS18980	257572..258003	-	432	WP_124740409.1	hypothetical protein	-
EHO51_RS18985	258030..258398	-	369	WP_124740410.1	antibiotic biosynthesis monooxygenase	-
EHO51_RS18990	258496..259005	+	510	WP_124740411.1	MarR family winged helix-turn-helix transcriptional regulator	-
EHO51_RS18995	259159..259386	+	228	WP_124740412.1	hypothetical protein	-
EHO51_RS19000	259383..259652	+	270	WP_124740413.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..347969	347969

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (5-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 18854.65 Da
• Isoelectric Point: 8.2542

>T115191 WP_124740407.1 NZ_CP034087:254603-255124 [Methylocystis rosea]
MTALLWEEAPLGKNHDRNAFDCGEDDLNVYLKRYARQNHESGGAKCFVATPRDAPARILGFYTLSPASIEFSRAPAIVTH
GLGRYEIPVYRVGRLAVDRSVQGLGLGGRLLLRAAERCVTVAQEVGGVALLIDAKSDGAAKWYESYGALRLLDAPLSLVL
PFSVVLEALKQRN

Nucleotide

• Length: 522 bp

>T115191 NZ_CP034087:254603-255124 [Methylocystis rosea]
ATGACCGCCCTCCTTTGGGAGGAAGCGCCGCTCGGCAAGAACCACGACCGTAACGCTTTCGATTGCGGCGAGGACGACCT
CAACGTCTATCTGAAACGCTACGCGCGCCAGAATCACGAGAGCGGCGGCGCGAAATGCTTCGTCGCCACGCCGCGCGACG
CGCCCGCGCGCATCCTCGGCTTCTACACGCTCAGCCCCGCCTCGATCGAATTCAGCCGCGCGCCCGCCATCGTAACGCAC
GGCCTCGGCCGCTATGAAATTCCGGTTTATCGCGTCGGCCGTCTCGCCGTTGATCGCAGCGTGCAAGGACTCGGCCTCGG
CGGACGGCTCTTGCTGCGCGCCGCCGAACGTTGCGTGACGGTCGCGCAAGAAGTAGGCGGCGTGGCGCTGTTGATCGACG
CGAAGAGCGACGGTGCGGCCAAATGGTACGAGAGCTATGGCGCGCTGCGTCTCCTCGATGCGCCGCTCTCCCTCGTGCTG
CCTTTCTCGGTCGTCCTCGAGGCTCTGAAACAGAGGAATTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10160.90 Da
• Isoelectric Point: 10.1684

>AT115191 WP_124740406.1 NZ_CP034087:254331-254606 [Methylocystis rosea]
MPREHVESGRVELRLKPEDKAVLARAAALERLDLTSFILRAALPRAKAVITEAEKLRLSERDSLRVLDLLENPPEPPARL
VRAAKAGFVLE

Nucleotide

• Length: 276 bp

>AT115191 NZ_CP034087:254331-254606 [Methylocystis rosea]
ATGCCCCGCGAACATGTCGAAAGCGGCCGGGTCGAGCTGCGGCTCAAACCCGAGGACAAGGCCGTGCTGGCGCGCGCCGC
CGCGCTCGAACGTCTCGACCTCACCAGCTTCATCCTGCGCGCCGCCCTGCCGCGCGCCAAGGCCGTCATCACGGAAGCCG
AAAAGCTGCGGCTATCGGAACGCGATTCTTTGCGCGTGCTCGATCTCTTGGAAAATCCGCCGGAGCCGCCGGCGCGCCTC
GTCCGCGCCGCCAAGGCGGGCTTCGTGCTCGAATGA