Detailed information of TA system TA115185

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): AbkB-shpB/BrnT-BrnA
• Location: 1685446..1685998
• Replicon: chromosome
• Accession: NZ_CP034086
• Organism: Methylocystis rosea strain GW6

Toxin (Protein)

• Gene name: AbkB
• Uniprot ID: -
• Locus tag: EHO51_RS08190
• Protein ID: WP_124738473.1
• Coordinates: 1685446..1685733 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: EHO51_RS08195
• Protein ID: WP_124738474.1
• Coordinates: 1685714..1685998 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHO51_RS08170	1680745..1681857	-	1113	WP_124738469.1	endonuclease/exonuclease/phosphatase family protein	-
EHO51_RS08175	1681946..1683508	-	1563	WP_124738470.1	MFS transporter	-
EHO51_RS08180	1683632..1684090	-	459	WP_124738471.1	hypothetical protein	-
EHO51_RS08185	1684231..1685385	+	1155	WP_124738472.1	23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN	-
EHO51_RS08190	1685446..1685733	+	288	WP_124738473.1	BrnT family toxin	Toxin
EHO51_RS08195	1685714..1685998	+	285	WP_124738474.1	BrnA antitoxin family protein	Antitoxin
EHO51_RS08200	1685973..1686524	-	552	WP_124738475.1	disulfide bond formation protein B	-
EHO51_RS08205	1686524..1687336	-	813	WP_124738476.1	C1 family peptidase	-
EHO51_RS08210	1687396..1687974	-	579	WP_018408582.1	DedA family protein	-
EHO51_RS08220	1688404..1689435	+	1032	WP_124738477.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-86)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (29-87)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10964.35 Da
• Isoelectric Point: 6.7564

>T115185 WP_124738473.1 NZ_CP034086:1685446-1685733 [Methylocystis rosea]
VKFEWDADKARRNAAKHGVSFEVARRVWDDPLHVILPDRFKDGEERWHAIGIVGGVALLVVVHCYPDEDDAEHVRIIGAR
KATPHERRRYEQEGV

Nucleotide

• Length: 288 bp

>T115185 NZ_CP034086:1685446-1685733 [Methylocystis rosea]
GTGAAATTCGAATGGGACGCTGACAAGGCGCGGCGCAATGCCGCCAAGCATGGGGTTTCATTCGAAGTCGCGCGACGCGT
TTGGGACGATCCCTTGCATGTCATCCTGCCCGACCGTTTCAAGGATGGCGAAGAACGTTGGCATGCGATCGGCATTGTCG
GCGGAGTGGCCTTGCTCGTTGTCGTGCATTGTTATCCTGACGAAGACGACGCCGAGCACGTACGGATCATCGGCGCCCGA
AAGGCGACGCCTCACGAGAGAAGACGCTATGAGCAAGAAGGAGTTTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 11254.79 Da
• Isoelectric Point: 9.5911

>AT115185 WP_124738474.1 NZ_CP034086:1685714-1685998 [Methylocystis rosea]
MSKKEFSPKERRELEKLAVLPDDEIDTKDIPEAPAENWAFAHRGAFYKPIKQPVNIRIDADVLDWFKRHTEGRGYQTEIN
RVLRRHVIEKERQR

Nucleotide

• Length: 285 bp

>AT115185 NZ_CP034086:1685714-1685998 [Methylocystis rosea]
ATGAGCAAGAAGGAGTTTAGCCCAAAGGAACGCCGCGAGCTTGAGAAGCTCGCCGTCTTGCCTGACGACGAGATCGACAC
GAAAGATATTCCTGAAGCTCCTGCCGAAAATTGGGCTTTCGCTCACCGCGGCGCGTTCTACAAGCCCATCAAACAGCCGG
TGAATATTCGCATCGATGCGGACGTGTTGGACTGGTTCAAGCGCCACACAGAGGGAAGAGGCTATCAAACGGAGATCAAC
CGCGTGTTGCGGCGCCATGTCATCGAGAAAGAAAGACAGCGCTGA