Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicA
Location 230418..230818 Replicon chromosome
Accession NZ_CP034086
Organism Methylocystis rosea strain GW6

Toxin (Protein)


Gene name hicA Uniprot ID J7QGK5
Locus tag EHO51_RS01015 Protein ID WP_014891040.1
Coordinates 230418..230612 (+) Length 65 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID J7QSK3
Locus tag EHO51_RS01020 Protein ID WP_014891039.1
Coordinates 230612..230818 (+) Length 69 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EHO51_RS00990 226061..226315 - 255 WP_124737324.1 hypothetical protein -
EHO51_RS00995 226777..227067 - 291 WP_124737325.1 hypothetical protein -
EHO51_RS01000 227405..228337 + 933 WP_124737326.1 polyprenyl synthetase family protein -
EHO51_RS01005 228350..229066 + 717 WP_124737327.1 DUF1345 domain-containing protein -
EHO51_RS01010 229074..230384 + 1311 WP_124737328.1 MFS transporter -
EHO51_RS01015 230418..230612 + 195 WP_014891040.1 type II toxin-antitoxin system HicA family toxin Toxin
EHO51_RS01020 230612..230818 + 207 WP_014891039.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EHO51_RS01025 230859..231476 - 618 WP_124737329.1 Uma2 family endonuclease -
EHO51_RS01035 231800..232039 - 240 WP_124737330.1 DUF1902 domain-containing protein -
EHO51_RS01040 232066..234081 - 2016 WP_124737331.1 RNA polymerase sigma factor RpoD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-66)


Sequences

Toxin        


Download         Length: 65 a.a.        Molecular weight: 7045.14 Da        Isoelectric Point: 10.7837

>T115182 WP_014891040.1 NZ_CP034086:230418-230612 [Methylocystis rosea]
MKVKDVIRLLEEDGWFLAATRGSHRQFKHPTKAGRATVAGKLSDDISPGTLNSILKQAGLKEKQ

Download         Length: 195 bp

>T115182 NZ_CP034086:230418-230612 [Methylocystis rosea]
ATGAAAGTAAAGGACGTGATCCGTTTGCTGGAGGAGGACGGCTGGTTTTTGGCCGCCACAAGAGGTAGCCATCGCCAGTT
CAAACACCCGACCAAGGCGGGTCGCGCAACCGTCGCGGGCAAGCTTTCAGACGATATTTCTCCGGGAACGCTGAACAGCA
TTCTTAAACAGGCAGGTCTGAAGGAGAAACAGTGA

Antitoxin


Download         Length: 69 a.a.        Molecular weight: 7292.17 Da        Isoelectric Point: 3.9327

>AT115182 WP_014891039.1 NZ_CP034086:230612-230818 [Methylocystis rosea]
MRYAVVIEKAGDNYSAYVPDLPGCIATGATISDVEQELREAIRFHIEGLESDGLPVPPPSAVAEYVEA

Download         Length: 207 bp

>AT115182 NZ_CP034086:230612-230818 [Methylocystis rosea]
ATGCGTTACGCAGTCGTGATCGAAAAGGCGGGCGATAATTATTCGGCCTATGTGCCGGACTTGCCGGGCTGCATCGCGAC
AGGAGCGACCATCTCCGACGTGGAGCAGGAACTCCGAGAAGCGATCCGATTCCATATTGAGGGACTGGAATCCGACGGTT
TACCCGTGCCACCGCCTAGCGCCGTTGCGGAATATGTCGAGGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G8M0L6

Antitoxin

Source ID Structure
AlphaFold DB A0A3G8M2T3

References