Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5888..6413 Replicon plasmid pR210-2-CTX
Accession NZ_CP034085
Organism Klebsiella pneumoniae subsp. pneumoniae strain R210-2

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag EHV09_RS29095 Protein ID WP_013023785.1
Coordinates 5888..6193 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag EHV09_RS29100 Protein ID WP_001568025.1
Coordinates 6195..6413 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EHV09_RS29065 1559..2185 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
EHV09_RS29070 2305..2484 + 180 WP_006788217.1 hypothetical protein -
EHV09_RS29075 2964..3758 - 795 WP_004197635.1 site-specific integrase -
EHV09_RS29080 3956..4972 - 1017 WP_017899884.1 hypothetical protein -
EHV09_RS29085 4983..5297 - 315 WP_053389906.1 hypothetical protein -
EHV09_RS29090 5324..5719 - 396 WP_017899885.1 hypothetical protein -
EHV09_RS29095 5888..6193 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
EHV09_RS29100 6195..6413 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
EHV09_RS29115 7044..7241 + 198 WP_017899886.1 hypothetical protein -
EHV09_RS29120 7231..7521 - 291 WP_013023783.1 hypothetical protein -
EHV09_RS29125 7518..8645 - 1128 WP_013023782.1 DUF4238 domain-containing protein -
EHV09_RS29130 8679..10271 - 1593 WP_015344964.1 hypothetical protein -
EHV09_RS29135 10478..11257 - 780 WP_013023780.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid qnrS1 / blaCTX-M-15 / blaTEM-1C - 1..113569 113569


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T115181 WP_013023785.1 NZ_CP034085:c6193-5888 [Klebsiella pneumoniae subsp. pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T115181 NZ_CP034085:c6193-5888 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT115181 WP_001568025.1 NZ_CP034085:c6413-6195 [Klebsiella pneumoniae subsp. pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT115181 NZ_CP034085:c6413-6195 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References