Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 92539..94107 Replicon plasmid pR210-2-VIM
Accession NZ_CP034084
Organism Klebsiella pneumoniae subsp. pneumoniae strain R210-2

Toxin (Protein)


Gene name hipA Uniprot ID A0A859R327
Locus tag EHV09_RS28750 Protein ID WP_024134513.1
Coordinates 92800..94107 (+) Length 436 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A859R2S1
Locus tag EHV09_RS28745 Protein ID WP_039272366.1
Coordinates 92539..92790 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EHV09_RS28715 88192..88917 + 726 WP_001553093.1 hypothetical protein -
EHV09_RS28720 88973..89425 + 453 WP_001553092.1 hypothetical protein -
EHV09_RS28725 89441..90043 - 603 WP_039272372.1 hypothetical protein -
EHV09_RS28730 90304..90585 + 282 WP_000575656.1 hypothetical protein -
EHV09_RS28735 90604..91167 + 564 WP_001132404.1 hypothetical protein -
EHV09_RS28740 91170..92174 + 1005 WP_039272368.1 tyrosine-type recombinase/integrase -
EHV09_RS28745 92539..92790 + 252 WP_039272366.1 helix-turn-helix domain-containing protein Antitoxin
EHV09_RS28750 92800..94107 + 1308 WP_024134513.1 type II toxin-antitoxin system HipA family toxin Toxin
EHV09_RS28755 94104..94598 + 495 WP_039272364.1 hypothetical protein -
EHV09_RS28760 94773..95711 - 939 WP_044596315.1 hypothetical protein -
EHV09_RS28765 95910..97550 + 1641 WP_031611419.1 PAAR domain-containing protein -
EHV09_RS28775 97922..98224 - 303 WP_031611417.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA14 / mph(A) / blaVIM-1 / aph(3')-XV / ant(3'')-Ia / catB2 / qacE / sul1 - 1..144158 144158


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-64)


Sequences

Toxin        


Download         Length: 436 a.a.        Molecular weight: 47950.12 Da        Isoelectric Point: 6.2444

>T115180 WP_024134513.1 NZ_CP034084:92800-94107 [Klebsiella pneumoniae subsp. pneumoniae]
MQTLTVAMNGEVVGTLYRDNKGAMSFQYAPEWISEPGSRAISMSLPLGHGRITGSEVFNFFSNLLPDSEAIIARMQARFR
VETTHPFDLLASVGRDCVGAIQLYSPDADIPTVMETLAEPLDEAQIEQLLEGYQEAPLGMAEDADFRISIAGAQEKTALL
WYQDRWQRPLGSTPTSHIFKLPIGKIVQNNIDLSESCENEWLCLRIAKAFGFDVANADLATFGKKKVLVVQRFDRRWSKS
GEWLLRLPQEDFCQALGIAPALKYESHGGPGIADAMKLLLGSRLAAKDREQFFKSQILFWMLAAIDGHGKNFSIFIEPAS
SFRMTPLYDVMSAFPIFKSGGIAAQKAKMGMALQGKNRQYHFAMIQPRHFISTAAHVGFPQDTAAQLMFDMATKTEEVVA
TVAAELPAGFPEHISSAIFEGLSSQAAKILKGVAK

Download         Length: 1308 bp

>T115180 NZ_CP034084:92800-94107 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCAGACGCTAACAGTGGCGATGAATGGTGAGGTCGTAGGAACACTGTACCGAGATAACAAAGGGGCCATGTCATTTCA
GTATGCCCCGGAGTGGATCTCGGAACCAGGTTCACGCGCCATTTCCATGTCACTGCCTTTGGGTCATGGCAGGATCACAG
GAAGTGAAGTCTTCAATTTCTTTAGCAACCTCCTACCCGACTCAGAGGCAATTATTGCCAGGATGCAGGCTAGGTTCAGA
GTTGAGACAACACATCCATTTGATTTGCTGGCGAGTGTTGGCCGGGATTGTGTTGGAGCTATCCAGCTCTATTCCCCCGA
TGCGGACATCCCAACTGTAATGGAGACGCTTGCAGAGCCTCTGGATGAAGCTCAGATTGAGCAACTGCTTGAAGGCTACC
AGGAAGCGCCCCTTGGGATGGCAGAGGATGCTGATTTTCGGATCTCGATTGCCGGAGCCCAAGAGAAAACTGCACTGCTT
TGGTATCAGGACCGCTGGCAACGCCCCCTGGGCAGCACCCCAACAAGCCATATTTTTAAGCTACCCATTGGTAAGATTGT
TCAAAATAACATCGATCTCAGTGAGAGTTGTGAGAATGAATGGCTCTGTTTGCGCATTGCAAAGGCCTTCGGTTTTGACG
TCGCCAATGCGGACCTAGCAACGTTCGGCAAGAAGAAGGTGCTTGTCGTTCAGCGTTTTGACCGGCGCTGGTCTAAATCC
GGCGAATGGCTTCTACGATTGCCCCAAGAAGACTTCTGCCAAGCTCTTGGGATCGCTCCTGCGCTCAAATACGAGTCACA
TGGAGGGCCAGGTATTGCTGACGCAATGAAGTTGTTGTTGGGGTCCAGACTTGCGGCTAAGGATAGAGAGCAATTTTTCA
AGTCGCAGATCCTATTCTGGATGTTGGCCGCGATTGATGGGCACGGGAAGAACTTCTCCATCTTCATTGAGCCTGCATCG
TCATTTAGGATGACCCCCCTCTACGATGTCATGTCTGCTTTTCCTATCTTCAAAAGCGGGGGAATTGCTGCTCAAAAGGC
CAAGATGGGGATGGCGCTCCAAGGCAAAAACCGCCAATACCATTTTGCGATGATCCAGCCTCGTCATTTTATCAGTACCG
CCGCCCATGTCGGGTTCCCACAAGATACCGCCGCTCAACTTATGTTCGACATGGCTACCAAGACAGAGGAGGTGGTTGCC
ACAGTAGCGGCTGAGCTGCCAGCCGGTTTCCCAGAGCACATCAGTAGCGCCATCTTCGAGGGGCTGTCCAGCCAGGCGGC
TAAGATCTTGAAGGGAGTAGCCAAATGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9369.79 Da        Isoelectric Point: 9.8930

>AT115180 WP_039272366.1 NZ_CP034084:92539-92790 [Klebsiella pneumoniae subsp. pneumoniae]
MKVTSPSLLAAAVRDQRKLSKLTQSEAAKQVGIKQTTVSDFELRPESTKLETLFKLLSALDLELHVVKRGSTLDDSKVWD
REW

Download         Length: 252 bp

>AT115180 NZ_CP034084:92539-92790 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAAGTGACATCTCCATCCTTGCTTGCGGCTGCGGTGCGTGACCAGCGCAAGCTCAGCAAACTAACGCAGTCAGAGGC
GGCGAAACAGGTCGGAATCAAGCAGACGACCGTCTCTGATTTTGAACTACGGCCAGAATCTACCAAGCTGGAGACGCTAT
TCAAGCTCCTTTCTGCACTGGATCTGGAGCTTCATGTCGTGAAGCGGGGTTCAACATTGGACGATAGTAAAGTCTGGGAT
AGGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A859R327

Antitoxin

Source ID Structure
AlphaFold DB A0A859R2S1

References