Detailed information of TA system TA115179

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 156019..156689
• Replicon: plasmid pR210-2-vir
• Accession: NZ_CP034083
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain R210-2

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: EHV09_RS27760
• Protein ID: WP_004213072.1
• Coordinates: 156019..156462 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: EHV09_RS27765
• Protein ID: WP_004213073.1
• Coordinates: 156459..156689 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHV09_RS27710	151430..151705	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
EHV09_RS27715	151768..152259	+	492	WP_011251327.1	DM13 domain-containing protein	-
EHV09_RS27720	152308..153228	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
EHV09_RS27730	153319..153725	+	407	Protein_162	GAF domain-containing protein	-
EHV09_RS30850	154240..154875	-	636	Protein_163	response regulator	-
EHV09_RS27750	155292..155585	+	294	Protein_164	transposase	-
EHV09_RS27755	155589..156005	+	417	Protein_165	restriction endonuclease	-
EHV09_RS27760	156019..156462	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
EHV09_RS27765	156459..156689	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EHV09_RS27770	157297..158430	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
EHV09_RS27775	158446..158739	+	294	WP_004213076.1	hypothetical protein	-
EHV09_RS27780	158729..158935	-	207	WP_004213077.1	hypothetical protein	-
EHV09_RS27785	159287..159577	+	291	WP_004213078.1	hypothetical protein	-
EHV09_RS27790	159567..160466	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..229648	229648

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T115179 WP_004213072.1 NZ_CP034083:c156462-156019 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T115179 NZ_CP034083:c156462-156019 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT115179 WP_004213073.1 NZ_CP034083:c156689-156459 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT115179 NZ_CP034083:c156689-156459 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620