Detailed information of TA system TA115161

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 327690..328276
• Replicon: chromosome
• Accession: NZ_CP034082
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain R210-2

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A330WAT7
• Locus tag: EHV09_RS01550
• Protein ID: WP_014906830.1
• Coordinates: 327908..328276 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: EHV09_RS01545
• Protein ID: WP_004174006.1
• Coordinates: 327690..327911 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHV09_RS01525	323847..324773	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
EHV09_RS01530	324770..326047	+	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
EHV09_RS01535	326044..326811	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
EHV09_RS01540	326813..327526	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
EHV09_RS01545	327690..327911	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
EHV09_RS01550	327908..328276	+	369	WP_014906830.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
EHV09_RS01555	328549..329865	+	1317	WP_004174008.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
EHV09_RS01560	329972..330859	+	888	WP_014906831.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
EHV09_RS01565	330856..331701	+	846	WP_004145129.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
EHV09_RS01570	331703..332773	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	322676..333510	10834

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (28-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13537.95 Da
• Isoelectric Point: 8.6410

>T115161 WP_014906830.1 NZ_CP034082:327908-328276 [Klebsiella pneumoniae subsp. pneumoniae]
MTLQIISAEEIILFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T115161 NZ_CP034082:327908-328276 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACTGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT115161 WP_004174006.1 NZ_CP034082:327690-327911 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT115161 NZ_CP034082:327690-327911 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A330WAT7

Antitoxin

• AlphaFold DB: A0A5E5YJY7