Detailed information of TA system TA115160

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE-Phd
• Location: 34494..35085
• Replicon: plasmid pPP1-3
• Accession: NZ_CP034081
• Organism: Pseudomonas syringae pv. pisi str. PP1

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: A0A0N8S0V9
• Locus tag: N032_RS28120
• Protein ID: WP_024650459.1
• Coordinates: 34494..34838 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: -
• Locus tag: N032_RS28125
• Protein ID: WP_024650458.1
• Coordinates: 34831..35085 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N032_RS28075	30406..30621	+	216	WP_032632253.1	hypothetical protein	-
N032_RS28085	31085..31372	+	288	WP_024650466.1	hypothetical protein	-
N032_RS28095	31828..32247	+	420	WP_024650465.1	hypothetical protein	-
N032_RS28100	32266..32505	+	240	WP_024650464.1	hypothetical protein	-
N032_RS28105	33136..33675	-	540	WP_024650461.1	single-stranded DNA-binding protein	-
N032_RS28110	33885..34139	+	255	WP_124733773.1	hypothetical protein	-
N032_RS28115	34126..34497	+	372	WP_024650460.1	hypothetical protein	-
N032_RS28120	34494..34838	-	345	WP_024650459.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
N032_RS28125	34831..35085	-	255	WP_024650458.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
N032_RS28130	35597..35902	+	306	WP_024650456.1	hypothetical protein	-
N032_RS28135	35911..36309	+	399	WP_024650455.1	DUF1311 domain-containing protein	-
N032_RS28140	36315..36599	+	285	WP_024650454.1	hypothetical protein	-
N032_RS28145	36635..36931	+	297	WP_024650453.1	hypothetical protein	-
N032_RS28150	37124..37750	+	627	WP_024650451.1	recombinase family protein	-
N032_RS28155	37858..38271	+	414	WP_024650450.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..39003	39003

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-98)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12953.81 Da
• Isoelectric Point: 4.3046

>T115160 WP_024650459.1 NZ_CP034081:c34838-34494 [Pseudomonas syringae pv. pisi str. PP1]
MSDIYIRYTDLAQSSLEDQVEHLSLYQDFTQAMLRVSNLIDTIQDKLLSAPLGYPISPQLSDLGVLHYRELNTDGYRVFY
EIVEADNAIVVKLVLGSKQSVESALIRYCLLQPI

Nucleotide

• Length: 345 bp

>T115160 NZ_CP034081:c34838-34494 [Pseudomonas syringae pv. pisi str. PP1]
ATGTCTGATATTTATATTCGCTACACCGATTTGGCACAAAGCAGCCTAGAAGATCAGGTTGAACACTTATCCTTGTATCA
AGACTTCACTCAGGCGATGCTACGCGTGAGCAATCTTATTGATACGATTCAAGATAAGCTACTATCTGCACCGCTGGGCT
ACCCGATAAGCCCCCAACTGAGCGACCTTGGGGTGCTGCACTATCGTGAGTTAAACACCGATGGGTATAGAGTTTTCTAT
GAGATTGTTGAAGCTGATAACGCTATCGTTGTGAAACTCGTCTTGGGCAGCAAGCAAAGTGTTGAAAGCGCTTTAATCCG
ATATTGCTTGCTGCAACCAATTTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9523.99 Da
• Isoelectric Point: 10.0634

>AT115160 WP_024650458.1 NZ_CP034081:c35085-34831 [Pseudomonas syringae pv. pisi str. PP1]
MRVETISYLKRHAADLPLDEPLVVTQNGVPAYVVESYAQRKRRDESIALVKLLAISSREYAQGKHCSADELKARLNTRLT
KKNV

Nucleotide

• Length: 255 bp

>AT115160 NZ_CP034081:c35085-34831 [Pseudomonas syringae pv. pisi str. PP1]
ATGCGAGTTGAAACCATTAGCTACCTGAAGCGCCACGCAGCCGATCTACCTCTCGACGAGCCGCTGGTGGTCACACAAAA
CGGTGTACCAGCTTACGTAGTGGAATCATACGCACAACGCAAGCGGCGAGACGAGTCGATCGCCCTGGTAAAGCTGTTGG
CAATCAGCTCCCGTGAGTACGCCCAAGGCAAGCATTGCTCCGCTGACGAACTCAAAGCACGTTTAAATACCAGACTTACA
AAGAAAAATGTCTGA

Structures

Toxin

• AlphaFold DB: A0A0N8S0V9