Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 21262..21809 Replicon plasmid pPP1-2
Accession NZ_CP034080
Organism Pseudomonas syringae pv. pisi str. PP1

Toxin (Protein)


Gene name mazF Uniprot ID Q48B48
Locus tag N032_RS27715 Protein ID WP_003319696.1
Coordinates 21262..21588 (-) Length 109 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7V8M172
Locus tag N032_RS27720 Protein ID WP_003319697.1
Coordinates 21585..21809 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N032_RS27690 19828..20100 - 273 WP_003348606.1 type II toxin-antitoxin system RelE/ParE family toxin -
N032_RS27695 20090..20275 - 186 WP_005730485.1 hypothetical protein -
N032_RS27700 20326..20604 - 279 WP_003320513.1 hypothetical protein -
N032_RS27715 21262..21588 - 327 WP_003319696.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
N032_RS27720 21585..21809 - 225 WP_003319697.1 antitoxin MazE family protein Antitoxin
N032_RS27725 22299..22931 + 633 WP_003381804.1 ParA family protein -
N032_RS27730 22967..23221 + 255 WP_003381801.1 hypothetical protein -
N032_RS27735 23646..24311 - 666 WP_024647998.1 LexA family transcriptional regulator -
N032_RS27740 24399..24629 + 231 WP_010215521.1 helix-turn-helix domain-containing protein -
N032_RS27745 24981..25679 + 699 WP_024650160.1 lytic transglycosylase domain-containing protein -
N032_RS27750 25679..26014 + 336 WP_024650161.1 hypothetical protein -
N032_RS27755 26027..26427 + 401 Protein_33 VirB3 family type IV secretion system protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..54993 54993


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-103)

Antitoxin

Antitoxin MazE-like

(8-72)


Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 11660.71 Da        Isoelectric Point: 8.0546

>T115159 WP_003319696.1 NZ_CP034080:c21588-21262 [Pseudomonas syringae pv. pisi str. PP1]
MMRGDLVTVAMQGDFGKPRPALVIQANQFSEHTSVTVLPVTSTLVAAPLLRITVQPTAENGLQKPSQVMLDKTMTLKREK
IGPTFGHIGVDSMVEVERCLAVFLGIAN

Download         Length: 327 bp

>T115159 NZ_CP034080:c21588-21262 [Pseudomonas syringae pv. pisi str. PP1]
ATGATGCGAGGTGACCTAGTCACCGTCGCCATGCAAGGTGACTTTGGTAAACCAAGACCCGCCTTGGTAATTCAGGCAAA
CCAATTCAGCGAACACACCAGCGTTACCGTGCTGCCAGTGACCAGCACCCTAGTCGCAGCACCGCTGCTGCGCATCACCG
TTCAGCCGACCGCAGAAAACGGCTTGCAGAAGCCTTCTCAGGTCATGCTAGACAAGACCATGACACTCAAGCGTGAAAAA
ATAGGTCCGACTTTTGGGCATATCGGAGTGGATTCAATGGTGGAGGTGGAGCGCTGTTTAGCCGTGTTCTTGGGTATCGC
CAATTGA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8471.61 Da        Isoelectric Point: 6.2008

>AT115159 WP_003319697.1 NZ_CP034080:c21809-21585 [Pseudomonas syringae pv. pisi str. PP1]
MPTSHVNARVQRHRDALRAAGLRPIQIWVPDTRRPDFAQECHRQCLLVAQADMANTYMQGLMDEALDDVEGWTP

Download         Length: 225 bp

>AT115159 NZ_CP034080:c21809-21585 [Pseudomonas syringae pv. pisi str. PP1]
ATGCCTACATCCCATGTAAATGCCCGCGTTCAACGCCATCGCGACGCGCTGCGTGCGGCTGGCCTACGGCCCATTCAGAT
TTGGGTTCCCGATACCCGGCGGCCAGACTTTGCACAGGAGTGCCATCGACAGTGCCTGTTGGTTGCTCAGGCAGACATGG
CCAATACCTATATGCAGGGGTTGATGGACGAAGCCTTAGACGATGTTGAGGGTTGGACGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V8S2J7

Antitoxin

Source ID Structure
AlphaFold DB A0A7V8M172

References