Detailed information of TA system TA115158

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 19828..20275
• Replicon: plasmid pPP1-2
• Accession: NZ_CP034080
• Organism: Pseudomonas syringae pv. pisi str. PP1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A3M4C5F0
• Locus tag: N032_RS27690
• Protein ID: WP_003348606.1
• Coordinates: 19828..20100 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A3M4C5H0
• Locus tag: N032_RS27695
• Protein ID: WP_005730485.1
• Coordinates: 20090..20275 (-)
• Length: 62 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N032_RS27690	19828..20100	-	273	WP_003348606.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
N032_RS27695	20090..20275	-	186	WP_005730485.1	hypothetical protein	Antitoxin
N032_RS27700	20326..20604	-	279	WP_003320513.1	hypothetical protein	-
N032_RS27715	21262..21588	-	327	WP_003319696.1	type II toxin-antitoxin system PemK/MazF family toxin	-
N032_RS27720	21585..21809	-	225	WP_003319697.1	antitoxin MazE family protein	-
N032_RS27725	22299..22931	+	633	WP_003381804.1	ParA family protein	-
N032_RS27730	22967..23221	+	255	WP_003381801.1	hypothetical protein	-
N032_RS27735	23646..24311	-	666	WP_024647998.1	LexA family transcriptional regulator	-
N032_RS27740	24399..24629	+	231	WP_010215521.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..54993	54993

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-89)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10199.67 Da
• Isoelectric Point: 5.0749

>T115158 WP_003348606.1 NZ_CP034080:c20100-19828 [Pseudomonas syringae pv. pisi str. PP1]
VLVEWLPQALEALEEIAGYLAERNPYAAEQMQATLEATVEALPLHPYIHRPGRVPGTREAVVHPNYLVVYRVGTERISIL
DVLHARQEYP

Nucleotide

• Length: 273 bp

>T115158 NZ_CP034080:c20100-19828 [Pseudomonas syringae pv. pisi str. PP1]
GTGCTAGTTGAGTGGCTTCCACAAGCTTTGGAGGCACTGGAGGAAATCGCCGGCTACCTAGCCGAGCGCAACCCTTATGC
TGCCGAGCAGATGCAGGCCACCCTTGAGGCCACGGTCGAGGCTCTCCCCCTGCACCCTTACATCCACCGTCCCGGCCGTG
TTCCAGGAACGCGCGAAGCGGTTGTGCATCCCAACTACCTGGTTGTCTACCGCGTCGGTACAGAGCGCATCAGCATCTTG
GACGTCCTGCACGCCCGCCAAGAGTACCCCTAG

Antitoxin

Protein

• Length: 62 a.a.
• Molecular weight: 7292.18 Da
• Isoelectric Point: 7.2040

>AT115158 WP_005730485.1 NZ_CP034080:c20275-20090 [Pseudomonas syringae pv. pisi str. PP1]
MSIQLDPRVSEFETQEQADNYDRWFRQRIEHSLADPRPPVPHDEAMARVRAMIEAKRRRAS

Nucleotide

• Length: 186 bp

>AT115158 NZ_CP034080:c20275-20090 [Pseudomonas syringae pv. pisi str. PP1]
ATGAGCATTCAACTTGACCCTCGCGTCTCGGAGTTCGAGACCCAGGAACAGGCCGACAATTACGACCGTTGGTTTCGCCA
GCGTATCGAGCACAGTTTGGCCGATCCGCGTCCACCCGTGCCGCACGATGAAGCAATGGCTCGCGTCAGAGCCATGATCG
AAGCAAAGCGCCGCCGTGCTAGTTGA

Structures

Toxin

• AlphaFold DB: A0A3M4C5F0

Antitoxin

• AlphaFold DB: A0A3M4C5H0