Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE-Phd
Location 12646..13257 Replicon plasmid pPP1-2
Accession NZ_CP034080
Organism Pseudomonas syringae pv. pisi str. PP1

Toxin (Protein)


Gene name PfiT Uniprot ID A0A7Z6UVP4
Locus tag N032_RS27680 Protein ID WP_003348598.1
Coordinates 12907..13257 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID Q48B86
Locus tag N032_RS27675 Protein ID WP_004662018.1
Coordinates 12646..12894 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N032_RS27650 8200..8487 + 288 WP_003348585.1 hypothetical protein -
N032_RS27655 8699..9340 + 642 WP_110768561.1 SOS response-associated peptidase -
N032_RS27660 9528..10103 + 576 WP_003348590.1 hypothetical protein -
N032_RS27665 11041..12003 - 963 WP_024650326.1 tyrosine-type recombinase/integrase -
N032_RS27670 12044..12406 - 363 WP_124733765.1 hypothetical protein -
N032_RS27675 12646..12894 + 249 WP_004662018.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
N032_RS27680 12907..13257 + 351 WP_003348598.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..54993 54993


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-101)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12994.80 Da        Isoelectric Point: 4.1953

>T115157 WP_003348598.1 NZ_CP034080:12907-13257 [Pseudomonas syringae pv. pisi str. PP1]
MNTFALRFTEVAQQSLEDQVEHLAVYQGFSSAAQRIDSLIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNTDGYRIFY
EVMDSDGINDIAVLLVLGGKQSVEQALIRYCLLQPM

Download         Length: 351 bp

>T115157 NZ_CP034080:12907-13257 [Pseudomonas syringae pv. pisi str. PP1]
ATGAATACGTTTGCACTACGCTTTACCGAGGTTGCCCAGCAAAGCCTCGAAGATCAGGTTGAACATCTGGCTGTCTATCA
GGGATTCTCATCAGCTGCTCAACGCATAGATTCCCTTATCGATGCCATCCAGGACAAGCTCCTGTCCACCCCGTTGGGCT
ACCCGGTCAGTCCACAGTTGAGTGAGCTGGGTGTACTGCACTATCGTGAGTTGAACACTGACGGATACCGGATCTTTTAT
GAGGTGATGGATTCTGATGGTATTAACGACATCGCCGTATTGCTCGTATTGGGTGGTAAACAGAGTGTTGAACAGGCACT
GATCCGCTACTGTCTGCTACAGCCTATGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9352.70 Da        Isoelectric Point: 9.8450

>AT115157 WP_004662018.1 NZ_CP034080:12646-12894 [Pseudomonas syringae pv. pisi str. PP1]
MRVETISYLKRNAADLPLDEPLVVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYSQGKHCSTDELKARLSRRFA
QK

Download         Length: 249 bp

>AT115157 NZ_CP034080:12646-12894 [Pseudomonas syringae pv. pisi str. PP1]
ATGCGAGTTGAAACAATAAGTTATCTGAAACGCAACGCAGCCGACCTGCCTCTCGATGAGCCATTGGTCGTCACGCAAAA
CGGTGTCCCGGCCTACGTGGTGGAGTCTTACGCTGATCGTAAGCGCCGCGATGAGTCAATTGCCTTGGTGAAGCTCCTGG
CAATCAGCTCCCGCGAGTACTCCCAAGGCAAGCACTGTTCCACTGACGAACTCAAAGCCCGATTGAGCAGGAGGTTTGCC
CAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z6UVP4

Antitoxin

Source ID Structure
AlphaFold DB A0A0N8T3U9

References