Detailed information of TA system TA115156

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/-
• Location: 1920..2398
• Replicon: plasmid pPP1-2
• Accession: NZ_CP034080
• Organism: Pseudomonas syringae pv. pisi str. PP1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: H1ZX33
• Locus tag: N032_RS27595
• Protein ID: WP_003348565.1
• Coordinates: 2105..2398 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2S4HLF9
• Locus tag: N032_RS27590
• Protein ID: WP_003348562.1
• Coordinates: 1920..2105 (+)
• Length: 62 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N032_RS27580	1..1314	+	1314	WP_032495804.1	replication initiation protein	-
N032_RS27585	1540..1833	+	294	WP_003348560.1	HU family DNA-binding protein	-
N032_RS27590	1920..2105	+	186	WP_003348562.1	hypothetical protein	Antitoxin
N032_RS27595	2105..2398	+	294	WP_003348565.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
N032_RS27600	2538..3065	+	528	WP_003348567.1	hypothetical protein	-
N032_RS27605	3062..3349	-	288	Protein_5	MobA/MobL family protein	-
N032_RS27610	3350..3631	+	282	Protein_6	S24 family peptidase	-
N032_RS27615	3609..3872	+	264	WP_080270888.1	DNA-directed DNA polymerase	-
N032_RS27620	4086..4499	-	414	WP_003348573.1	DUF3757 domain-containing protein	-
N032_RS27625	4924..5964	+	1041	WP_080270889.1	DUF4113 domain-containing protein	-
N032_RS27630	6118..6405	+	288	WP_074454502.1	hypothetical protein	-
N032_RS27635	6499..6909	+	411	WP_005782529.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..54993	54993

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11136.84 Da
• Isoelectric Point: 6.2150

>T115156 WP_003348565.1 NZ_CP034080:2105-2398 [Pseudomonas syringae pv. pisi str. PP1]
MLPIFWLESADNDLAAIIEYIGSRDIAAAERLWQRLRGIVLPLSEHPYLYAISDRVPGMREIVAHPNYLVFYRVTSTRIE
VVNVVHARQEYPQTGLA

Nucleotide

• Length: 294 bp

>T115156 NZ_CP034080:2105-2398 [Pseudomonas syringae pv. pisi str. PP1]
ATGCTACCCATTTTCTGGCTAGAATCAGCGGACAACGATTTGGCGGCAATAATCGAATACATAGGTTCACGTGATATAGC
TGCTGCCGAAAGGTTATGGCAGCGCTTGCGTGGCATCGTTCTGCCCCTTTCAGAACATCCTTATCTGTATGCGATAAGTG
ACCGCGTCCCAGGAATGCGCGAGATCGTTGCCCATCCTAACTACCTGGTGTTTTATCGTGTCACCTCAACGCGCATAGAA
GTGGTGAACGTCGTCCACGCACGGCAAGAATATCCCCAAACTGGACTAGCGTAG

Antitoxin

Protein

• Length: 62 a.a.
• Molecular weight: 7134.04 Da
• Isoelectric Point: 6.4675

>AT115156 WP_003348562.1 NZ_CP034080:1920-2105 [Pseudomonas syringae pv. pisi str. PP1]
MIDRSPIVSEFETEELEANYTAWLRAKVEASLADSRPAIPHDEVERRMAERLARLRHRRAS

Nucleotide

• Length: 186 bp

>AT115156 NZ_CP034080:1920-2105 [Pseudomonas syringae pv. pisi str. PP1]
ATGATTGATCGTTCGCCTATCGTTTCGGAGTTTGAAACTGAAGAGCTAGAGGCCAATTACACCGCCTGGCTACGCGCGAA
GGTGGAGGCCAGTCTGGCCGACTCGCGCCCTGCTATCCCGCATGATGAAGTCGAGAGAAGGATGGCAGAGCGGCTGGCAC
GATTGCGCCATCGCCGAGCGTCCTGA

Structures

Toxin

• AlphaFold DB: A0A193SFZ9

Antitoxin

• AlphaFold DB: A0A2S4HLF9