Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/-
Location 1920..2398 Replicon plasmid pPP1-2
Accession NZ_CP034080
Organism Pseudomonas syringae pv. pisi str. PP1

Toxin (Protein)


Gene name relE Uniprot ID H1ZX33
Locus tag N032_RS27595 Protein ID WP_003348565.1
Coordinates 2105..2398 (+) Length 98 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A2S4HLF9
Locus tag N032_RS27590 Protein ID WP_003348562.1
Coordinates 1920..2105 (+) Length 62 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N032_RS27580 1..1314 + 1314 WP_032495804.1 replication initiation protein -
N032_RS27585 1540..1833 + 294 WP_003348560.1 HU family DNA-binding protein -
N032_RS27590 1920..2105 + 186 WP_003348562.1 hypothetical protein Antitoxin
N032_RS27595 2105..2398 + 294 WP_003348565.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
N032_RS27600 2538..3065 + 528 WP_003348567.1 hypothetical protein -
N032_RS27605 3062..3349 - 288 Protein_5 MobA/MobL family protein -
N032_RS27610 3350..3631 + 282 Protein_6 S24 family peptidase -
N032_RS27615 3609..3872 + 264 WP_080270888.1 DNA-directed DNA polymerase -
N032_RS27620 4086..4499 - 414 WP_003348573.1 DUF3757 domain-containing protein -
N032_RS27625 4924..5964 + 1041 WP_080270889.1 DUF4113 domain-containing protein -
N032_RS27630 6118..6405 + 288 WP_074454502.1 hypothetical protein -
N032_RS27635 6499..6909 + 411 WP_005782529.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..54993 54993


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 98 a.a.        Molecular weight: 11136.84 Da        Isoelectric Point: 6.2150

>T115156 WP_003348565.1 NZ_CP034080:2105-2398 [Pseudomonas syringae pv. pisi str. PP1]
MLPIFWLESADNDLAAIIEYIGSRDIAAAERLWQRLRGIVLPLSEHPYLYAISDRVPGMREIVAHPNYLVFYRVTSTRIE
VVNVVHARQEYPQTGLA

Download         Length: 294 bp

>T115156 NZ_CP034080:2105-2398 [Pseudomonas syringae pv. pisi str. PP1]
ATGCTACCCATTTTCTGGCTAGAATCAGCGGACAACGATTTGGCGGCAATAATCGAATACATAGGTTCACGTGATATAGC
TGCTGCCGAAAGGTTATGGCAGCGCTTGCGTGGCATCGTTCTGCCCCTTTCAGAACATCCTTATCTGTATGCGATAAGTG
ACCGCGTCCCAGGAATGCGCGAGATCGTTGCCCATCCTAACTACCTGGTGTTTTATCGTGTCACCTCAACGCGCATAGAA
GTGGTGAACGTCGTCCACGCACGGCAAGAATATCCCCAAACTGGACTAGCGTAG

Antitoxin


Download         Length: 62 a.a.        Molecular weight: 7134.04 Da        Isoelectric Point: 6.4675

>AT115156 WP_003348562.1 NZ_CP034080:1920-2105 [Pseudomonas syringae pv. pisi str. PP1]
MIDRSPIVSEFETEELEANYTAWLRAKVEASLADSRPAIPHDEVERRMAERLARLRHRRAS

Download         Length: 186 bp

>AT115156 NZ_CP034080:1920-2105 [Pseudomonas syringae pv. pisi str. PP1]
ATGATTGATCGTTCGCCTATCGTTTCGGAGTTTGAAACTGAAGAGCTAGAGGCCAATTACACCGCCTGGCTACGCGCGAA
GGTGGAGGCCAGTCTGGCCGACTCGCGCCCTGCTATCCCGCATGATGAAGTCGAGAGAAGGATGGCAGAGCGGCTGGCAC
GATTGCGCCATCGCCGAGCGTCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A193SFZ9

Antitoxin

Source ID Structure
AlphaFold DB A0A2S4HLF9

References