Detailed information of TA system TA115155

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 10111..10691
• Replicon: plasmid pPP1-1
• Accession: NZ_CP034079
• Organism: Pseudomonas syringae pv. pisi str. PP1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: Q48B35
• Locus tag: N032_RS27290
• Protein ID: WP_011282511.1
• Coordinates: 10392..10691 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: N032_RS27285
• Protein ID: WP_003348777.1
• Coordinates: 10111..10395 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N032_RS27255	5381..6211	-	831	WP_005770909.1	type III effector	-
N032_RS27260	6358..7398	+	1041	WP_080270893.1	Y-family DNA polymerase	-
N032_RS27265	7599..7793	+	195	WP_054096784.1	hypothetical protein	-
N032_RS27275	8475..9704	-	1230	WP_124733760.1	IS91 family transposase	-
N032_RS27280	9685..9966	-	282	WP_024647929.1	hypothetical protein	-
N032_RS27285	10111..10395	-	285	WP_003348777.1	putative addiction module antidote protein	Antitoxin
N032_RS27290	10392..10691	-	300	WP_011282511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
N032_RS27295	10863..11150	+	288	WP_041924941.1	hypothetical protein	-
N032_RS27300	11202..11618	+	417	WP_003348781.1	hypothetical protein	-
N032_RS27310	12013..12666	+	654	WP_003348786.1	AAA family ATPase	-
N032_RS27315	12755..13006	+	252	WP_003348789.1	ribbon-helix-helix protein, CopG family	-
N032_RS27320	13274..13915	+	642	WP_058398749.1	SOS response-associated peptidase family protein	-
N032_RS27325	14196..14873	+	678	WP_024647926.1	site-specific integrase	-
N032_RS27330	14952..15197	+	246	WP_003348793.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..62150	62150
	-	flank	IS/Tn	-	-	8475..9683	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Uncharacterized protein HI1420 (6-91)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11168.79 Da
• Isoelectric Point: 10.2231

>T115155 WP_011282511.1 NZ_CP034079:c10691-10392 [Pseudomonas syringae pv. pisi str. PP1]
MTTIKQTSTYMAWERRLKDQKAKAAIAARIFRVANGLMGDVSPVGQGVSELRIHVGPGYRVYFQQRGDELILLLCGGDKS
SQSRDIETAKRLADQWRQE

Nucleotide

• Length: 300 bp

>T115155 NZ_CP034079:c10691-10392 [Pseudomonas syringae pv. pisi str. PP1]
ATGACGACGATAAAACAGACATCTACCTATATGGCTTGGGAACGCAGGCTCAAAGACCAAAAGGCCAAAGCTGCAATTGC
CGCTCGTATTTTTAGAGTTGCAAATGGCCTGATGGGTGATGTCTCGCCAGTAGGGCAGGGCGTGAGTGAGCTACGGATAC
ACGTTGGCCCTGGTTACCGTGTTTATTTCCAGCAGCGAGGCGATGAACTGATCTTGCTGCTGTGCGGTGGTGACAAGAGC
AGCCAATCCCGAGATATCGAAACAGCAAAGAGACTGGCTGATCAATGGAGGCAAGAATGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10263.80 Da
• Isoelectric Point: 5.0916

>AT115155 WP_003348777.1 NZ_CP034079:c10395-10111 [Pseudomonas syringae pv. pisi str. PP1]
MKEQIFDYDPAAALDGPEAIAIFISDAFETGDAEYIAKAMGVVARAKGMSELARETGLSREQLYRSFSSKGNPTLKTMLT
VMRALGVDMTARMH

Nucleotide

• Length: 285 bp

>AT115155 NZ_CP034079:c10395-10111 [Pseudomonas syringae pv. pisi str. PP1]
ATGAAAGAACAAATTTTCGATTACGACCCAGCAGCAGCCCTTGACGGGCCTGAGGCAATCGCTATTTTTATCTCTGACGC
ATTTGAGACGGGGGATGCGGAGTACATCGCGAAGGCTATGGGGGTTGTCGCAAGGGCCAAAGGGATGAGCGAGCTTGCGC
GTGAGACAGGTCTTTCTCGAGAGCAGCTTTACAGATCGTTCAGTAGCAAGGGCAATCCAACCTTAAAAACCATGTTGACG
GTAATGAGAGCCCTGGGCGTGGACATGACAGCTCGAATGCACTGA

Structures

Toxin

• AlphaFold DB: A0A3M3YY44