Detailed information of TA system TA115134

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 700600..701441
• Replicon: chromosome
• Accession: NZ_CP034077
• Organism: Klebsiella pneumoniae strain KP17-16

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1M0WJM1
• Locus tag: EHE18_RS03540
• Protein ID: WP_000854822.1
• Coordinates: 701058..701441 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1M0CWL2
• Locus tag: EHE18_RS03535
• Protein ID: WP_053271974.1
• Coordinates: 700600..700968 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHE18_RS03505	695895..698018	+	2124	Protein_674	Ag43/Cah family autotransporter adhesin	-
EHE18_RS03515	698345..699163	+	819	WP_001234702.1	DUF945 domain-containing protein	-
EHE18_RS03520	699254..699739	+	486	WP_000214317.1	antirestriction protein	-
EHE18_RS03525	699754..700230	+	477	WP_001186747.1	RadC family protein	-
EHE18_RS03530	700299..700520	+	222	WP_000692312.1	DUF987 domain-containing protein	-
EHE18_RS03535	700600..700968	+	369	WP_053271974.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EHE18_RS03540	701058..701441	+	384	WP_000854822.1	type IV toxin-antitoxin system toxin CbtA	Toxin
EHE18_RS03545	701432..701920	+	489	WP_001177592.1	hypothetical protein	-
EHE18_RS03550	701887..702129	+	243	WP_166635458.1	DUF957 domain-containing protein	-
EHE18_RS03555	702226..703071	+	846	WP_001280435.1	DUF4942 domain-containing protein	-
EHE18_RS03565	703370..703876	+	507	WP_002917636.1	G/U mismatch-specific DNA glycosylase	-
EHE18_RS03570	703976..705817	-	1842	WP_004174339.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	aac(3)-IId	fimD / fimF / fimG / rfaE	674537..745158	70621

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14295.32 Da
• Isoelectric Point: 8.2830

>T115134 WP_000854822.1 NZ_CP034077:701058-701441 [Klebsiella pneumoniae]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHQESKRCN

Nucleotide

• Length: 384 bp

>T115134 NZ_CP034077:701058-701441 [Klebsiella pneumoniae]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCAGGAGTCGAAACGATGCAACTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13679.41 Da
• Isoelectric Point: 6.4758

>AT115134 WP_053271974.1 NZ_CP034077:700600-700968 [Klebsiella pneumoniae]
VSDTFSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLTDRAGIRGRFSDADANHLDQAFPLLMKQLELMLTSS
ELNPHRQNTVTLYVKGLTCHADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT115134 NZ_CP034077:700600-700968 [Klebsiella pneumoniae]
GTGTCAGACACATTCTCCGGGACAACGCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGTACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTACAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCGGATGCGAACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCAGT
GAACTGAATCCCCACCGTCAGAATACCGTCACGCTGTATGTTAAAGGGCTGACCTGCCATGCGGACACCCTCGGCAGCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A1M0WJM1

Antitoxin

• AlphaFold DB: A0A1M0CWL2