Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2662900..2663554 Replicon chromosome
Accession NZ_CP034067
Organism Shigella sonnei strain FDAARGOS_524

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag EGX92_RS13910 Protein ID WP_000244777.1
Coordinates 2663147..2663554 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EGX92_RS13905 Protein ID WP_000354046.1
Coordinates 2662900..2663166 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGX92_RS13880 (2658069) 2658069..2658812 + 744 WP_000951937.1 SDR family oxidoreductase -
EGX92_RS13885 (2658869) 2658869..2660302 - 1434 WP_005137189.1 6-phospho-beta-glucosidase BglA -
EGX92_RS13890 (2660347) 2660347..2660658 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
EGX92_RS13895 (2660822) 2660822..2661481 + 660 WP_000250274.1 hemolysin III family protein -
EGX92_RS13900 (2661677) 2661677..2662657 - 981 WP_000886060.1 tRNA-modifying protein YgfZ -
EGX92_RS13905 (2662900) 2662900..2663166 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EGX92_RS13910 (2663147) 2663147..2663554 + 408 WP_000244777.1 protein YgfX Toxin
EGX92_RS13915 (2663594) 2663594..2664115 - 522 WP_001055874.1 flavodoxin FldB -
EGX92_RS13920 (2664227) 2664227..2665123 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EGX92_RS13925 (2665148) 2665148..2665858 + 711 WP_000715206.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EGX92_RS13930 (2665864) 2665864..2667597 + 1734 WP_000813167.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T115101 WP_000244777.1 NZ_CP034067:2663147-2663554 [Shigella sonnei]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T115101 NZ_CP034067:2663147-2663554 [Shigella sonnei]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT115101 WP_000354046.1 NZ_CP034067:2662900-2663166 [Shigella sonnei]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT115101 NZ_CP034067:2662900-2663166 [Shigella sonnei]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References