Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 1298415..1298912 Replicon chromosome
Accession NZ_CP034067
Organism Shigella sonnei strain FDAARGOS_524

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag EGX92_RS07035 Protein ID WP_001173343.1
Coordinates 1298415..1298588 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag EGX92_RS07040 Protein ID WP_000398619.1
Coordinates 1298616..1298912 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGX92_RS07010 (1293567) 1293567..1294723 + 1157 WP_087879933.1 IS3-like element IS600 family transposase -
EGX92_RS07020 (1294769) 1294769..1295026 + 258 Protein_1233 lysine decarboxylase LdcC -
EGX92_RS07025 (1295085) 1295085..1296542 + 1458 WP_000856831.1 dipeptide/tripeptide permease DtpC -
EGX92_RS07030 (1296779) 1296779..1298296 + 1518 WP_001295086.1 lysine--tRNA ligase -
EGX92_RS07035 (1298415) 1298415..1298588 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
EGX92_RS07040 (1298616) 1298616..1298912 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
EGX92_RS07045 (1299139) 1299139..1299411 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
EGX92_RS07050 (1299423) 1299423..1299653 - 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
EGX92_RS07055 (1299834) 1299834..1301465 + 1632 WP_001216495.1 sensor histidine kinase -
EGX92_RS07060 (1301462) 1301462..1302181 + 720 WP_000611285.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T115099 WP_001173343.1 NZ_CP034067:c1298588-1298415 [Shigella sonnei]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T115099 NZ_CP034067:c1298588-1298415 [Shigella sonnei]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT115099 WP_000398619.1 NZ_CP034067:c1298912-1298616 [Shigella sonnei]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT115099 NZ_CP034067:c1298912-1298616 [Shigella sonnei]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References