Detailed information of TA system TA115097

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 610106..610943
• Replicon: chromosome
• Accession: NZ_CP034067
• Organism: Shigella sonnei strain FDAARGOS_524

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: EGX92_RS03685
• Protein ID: WP_000227784.1
• Coordinates: 610401..610943 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: EGX92_RS03680
• Protein ID: WP_001297137.1
• Coordinates: 610106..610417 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX92_RS03655 (605126)	605126..606073	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
EGX92_RS03660 (606095)	606095..608086	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
EGX92_RS03665 (608076)	608076..608690	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
EGX92_RS03670 (608690)	608690..609019	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
EGX92_RS03675 (609031)	609031..609921	+	891	WP_000971327.1	heme o synthase	-
EGX92_RS03680 (610106)	610106..610417	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
EGX92_RS03685 (610401)	610401..610943	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
EGX92_RS03690 (610999)	610999..611565	-	567	Protein_577	SEL1-like repeat protein	-
EGX92_RS03695 (611599)	611599..612296	+	698	WP_237158184.1	IS1-like element IS1A family transposase	-
EGX92_RS03700 (612313)	612313..612711	-	399	Protein_579	SEL1-like repeat protein	-
EGX92_RS03710 (613896)	613896..615260	+	1365	WP_001000971.1	MFS transporter	-
EGX92_RS03715 (615388)	615388..615879	-	492	WP_001138904.1	nucleotide binding protein YajQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T115097 WP_000227784.1 NZ_CP034067:610401-610943 [Shigella sonnei]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T115097 NZ_CP034067:610401-610943 [Shigella sonnei]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT115097 WP_001297137.1 NZ_CP034067:610106-610417 [Shigella sonnei]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT115097 NZ_CP034067:610106-610417 [Shigella sonnei]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3