Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 572630..573248 Replicon chromosome
Accession NZ_CP034067
Organism Shigella sonnei strain FDAARGOS_524

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag EGX92_RS03495 Protein ID WP_001291435.1
Coordinates 573030..573248 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A0H8V4S8
Locus tag EGX92_RS03490 Protein ID WP_000344804.1
Coordinates 572630..573004 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGX92_RS03480 (567719) 567719..568912 + 1194 WP_011310152.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
EGX92_RS03485 (568935) 568935..572084 + 3150 WP_001132464.1 efflux RND transporter permease AcrB -
EGX92_RS03490 (572630) 572630..573004 + 375 WP_000344804.1 Hha toxicity modulator TomB Antitoxin
EGX92_RS03495 (573030) 573030..573248 + 219 WP_001291435.1 HHA domain-containing protein Toxin
EGX92_RS03500 (573419) 573419..573970 + 552 WP_000102564.1 maltose O-acetyltransferase -
EGX92_RS03505 (574086) 574086..574556 + 471 WP_000136192.1 YlaC family protein -
EGX92_RS03510 (574720) 574720..576268 + 1549 Protein_541 cyclic-guanylate-specific phosphodiesterase PdeB -
EGX92_RS03515 (576310) 576310..576663 - 354 WP_000878140.1 DUF1428 domain-containing protein -
EGX92_RS03525 (577042) 577042..577353 + 312 WP_000409911.1 MGMT family protein -
EGX92_RS03530 (577384) 577384..577956 - 573 WP_000779812.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T115096 WP_001291435.1 NZ_CP034067:573030-573248 [Shigella sonnei]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T115096 NZ_CP034067:573030-573248 [Shigella sonnei]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14615.43 Da        Isoelectric Point: 4.6232

>AT115096 WP_000344804.1 NZ_CP034067:572630-573004 [Shigella sonnei]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIETFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT115096 NZ_CP034067:572630-573004 [Shigella sonnei]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGAGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H8V4S8

References