Detailed information of TA system TA115095

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 9554..10352
• Replicon: plasmid unnamed1
• Accession: NZ_CP034066
• Organism: Shigella sonnei strain FDAARGOS_524

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: EGX92_RS00065
• Protein ID: WP_032187665.1
• Coordinates: 9831..10352 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A141BRA2
• Locus tag: EGX92_RS00060
• Protein ID: WP_001711191.1
• Coordinates: 9554..9823 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX92_RS00035 (EGX92_00035)	4995..6335	-	1341	WP_000137333.1	DnaB-like helicase C-terminal domain-containing protein	-
EGX92_RS00040 (EGX92_00040)	6379..7119	-	741	WP_072651243.1	hypothetical protein	-
EGX92_RS00045 (EGX92_00045)	7492..8169	+	678	WP_072652150.1	hypothetical protein	-
EGX92_RS00050 (EGX92_00050)	8208..8561	-	354	WP_162521425.1	hypothetical protein	-
EGX92_RS00055 (EGX92_00055)	8567..9235	-	669	WP_053902532.1	division plane positioning ATPase MipZ	-
EGX92_RS00060 (EGX92_00060)	9554..9823	+	270	WP_001711191.1	DUF1778 domain-containing protein	Antitoxin
EGX92_RS00065 (EGX92_00065)	9831..10352	+	522	WP_032187665.1	GNAT family N-acetyltransferase	Toxin
EGX92_RS00075 (EGX92_00075)	10521..10772	-	252	WP_077108702.1	hypothetical protein	-
EGX92_RS00080 (EGX92_00080)	10774..11466	-	693	WP_032084069.1	membrane protein	-
EGX92_RS00085 (EGX92_00085)	11480..11803	-	324	WP_000064175.1	hypothetical protein	-
EGX92_RS00090 (EGX92_00090)	11878..12660	-	783	WP_077108701.1	receptor-recognizing protein	-
EGX92_RS25505	12727..13128	-	402	WP_171729182.1	tail fiber domain-containing protein	-
EGX92_RS26120 (EGX92_00100)	14185..14703	-	519	Protein_18	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..109082	109082

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (48-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19345.11 Da
• Isoelectric Point: 7.7881

>T115095 WP_032187665.1 NZ_CP034066:9831-10352 [Shigella sonnei]
VSNTTIEIFSGENDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEEIPKVLGYYTLSGSCFEKETLPSKSQQ
KKVPYRNVPSITLGRLALDKSLQGQGLGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKARAFYKSLGFIQLVGNNDRSL
FYPTKSIEKLFEE

Nucleotide

• Length: 522 bp

>T115095 NZ_CP034066:9831-10352 [Shigella sonnei]
GTGAGCAATACGACGATCGAGATTTTCTCTGGAGAGAACGATTATGATCTAAACGGTTTTGATTGCGGCGAAGAGTCACT
AAACGCATTTTTGACCAACCACTTAAAAAGACAGCATGAAGGAAAAATTCTTCGCGCTTATGTGCTTTGCACTAAAGAAG
AAATACCAAAGGTATTAGGTTATTACACTTTGTCAGGCAGCTGCTTTGAGAAAGAAACCTTACCTTCAAAGAGTCAGCAA
AAGAAAGTGCCTTATCGGAATGTCCCAAGTATCACTTTAGGCAGACTGGCTTTGGACAAATCTCTTCAAGGTCAGGGGCT
TGGCTCAATGCTTGTAACTCACGCAATGCGTGTTGTGTACAATGCATCTCTTGCGGTAGGCATTCATGGGCTTTTCGTTG
AGGCGTTGAACGATAAAGCCAGGGCGTTTTATAAAAGTTTGGGCTTTATCCAGCTGGTTGGTAACAACGATCGTTCTTTG
TTCTATCCAACAAAATCTATCGAAAAATTGTTCGAAGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10054.53 Da
• Isoelectric Point: 8.7353

>AT115095 WP_001711191.1 NZ_CP034066:9554-9823 [Shigella sonnei]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLSEESWNLVMDAISNPPAPNDKLKR
AANRLKSME

Nucleotide

• Length: 270 bp

>AT115095 NZ_CP034066:9554-9823 [Shigella sonnei]
ATGTCAGCACTAAAAAAGCAGCGCATCGATTTGAGATTAACCGATGACGATAAAAGCATCATCGAGGAAGCTGCCGCAAT
GTCTAACCAGAGCATTACTCAGTTTATGGTTAGCAGTGCATCTGAACGTGCCGTGAAAGTTATAGAGCAACACCGTAGAC
TAGTTCTAAGTGAAGAGTCCTGGAATCTGGTTATGGATGCTATAAGCAATCCTCCGGCACCGAACGACAAGCTGAAACGA
GCTGCCAATCGTCTGAAAAGCATGGAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A141BRA2