Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | hicAB/- |
| Location | 18358..18950 | Replicon | plasmid unnamed2 |
| Accession | NZ_CP034061 | ||
| Organism | Shigella flexneri strain FDAARGOS_535 | ||
Toxin (Protein)
| Gene name | hicA | Uniprot ID | A0A1I9ZJC5 |
| Locus tag | EGX88_RS25040 | Protein ID | WP_000513532.1 |
| Coordinates | 18702..18950 (-) | Length | 83 a.a. |
Antitoxin (Protein)
| Gene name | hicB | Uniprot ID | A0A1I9ZJ49 |
| Locus tag | EGX88_RS25035 | Protein ID | WP_000681609.1 |
| Coordinates | 18358..18705 (-) | Length | 116 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EGX88_RS24995 (EGX88_25010) | 13649..13990 | - | 342 | WP_000730722.1 | plasmid mobilization relaxosome protein MobC | - |
| EGX88_RS25000 (EGX88_25015) | 14359..14760 | + | 402 | WP_021575837.1 | hypothetical protein | - |
| EGX88_RS25005 (EGX88_25020) | 14778..15530 | + | 753 | WP_000797966.1 | conjugal transfer protein TraL | - |
| EGX88_RS25010 (EGX88_25025) | 15532..16239 | + | 708 | WP_000108794.1 | hypothetical protein | - |
| EGX88_RS26425 | 16259..16381 | + | 123 | Protein_15 | hypothetical protein | - |
| EGX88_RS25015 (EGX88_25030) | 16376..16699 | + | 324 | WP_223666488.1 | hypothetical protein | - |
| EGX88_RS25020 (EGX88_25035) | 16701..17027 | + | 327 | WP_000206697.1 | hypothetical protein | - |
| EGX88_RS25025 (EGX88_25040) | 17287..17586 | - | 300 | WP_000466320.1 | type II toxin-antitoxin system HicB family antitoxin | - |
| EGX88_RS26430 | 17827..18270 | + | 444 | WP_023223351.1 | hypothetical protein | - |
| EGX88_RS25035 (EGX88_25050) | 18358..18705 | - | 348 | WP_000681609.1 | type II toxin-antitoxin system HicB family antitoxin | Antitoxin |
| EGX88_RS25040 (EGX88_25055) | 18702..18950 | - | 249 | WP_000513532.1 | type II toxin-antitoxin system HicA family toxin | Toxin |
| EGX88_RS25045 (EGX88_25060) | 19415..19693 | - | 279 | WP_000545937.1 | hypothetical protein | - |
| EGX88_RS25060 (EGX88_25075) | 20242..20493 | - | 252 | WP_001132407.1 | transcriptional regulator | - |
| EGX88_RS25065 (EGX88_25080) | 20483..20743 | - | 261 | WP_000702246.1 | hypothetical protein | - |
| EGX88_RS25070 (EGX88_25085) | 20795..21019 | - | 225 | WP_000814384.1 | hypothetical protein | - |
| EGX88_RS25075 (EGX88_25090) | 21117..21326 | - | 210 | WP_000866037.1 | hypothetical protein | - |
| EGX88_RS25080 (EGX88_25095) | 21367..21690 | - | 324 | WP_000427402.1 | hypothetical protein | - |
| EGX88_RS25085 (EGX88_25105) | 22269..22598 | - | 330 | WP_000855121.1 | hypothetical protein | - |
| EGX88_RS25095 (EGX88_25115) | 23196..23861 | + | 666 | WP_000532148.1 | division plane positioning ATPase MipZ | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | - | 1..57818 | 57818 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 83 a.a. Molecular weight: 9378.03 Da Isoelectric Point: 10.4800
>T115093 WP_000513532.1 NZ_CP034061:c18950-18702 [Shigella flexneri]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL
Download Length: 249 bp
>T115093 NZ_CP034061:c18950-18702 [Shigella flexneri]
ATGGGGAAAGCAGATAAGCTACTGGCAAAATTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTCGTTGTTTTGTT
TTCTTCTCTAGGATATGTCAAAAAGGAAATGCAGGGATCAAGAGTGCGATTTTTCAATGCTGAAATTAATCACACAATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAGCTATTAAACAGAACTTGAAAGAGGCTGGG
GTTTTATGA
ATGGGGAAAGCAGATAAGCTACTGGCAAAATTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTCGTTGTTTTGTT
TTCTTCTCTAGGATATGTCAAAAAGGAAATGCAGGGATCAAGAGTGCGATTTTTCAATGCTGAAATTAATCACACAATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAGCTATTAAACAGAACTTGAAAGAGGCTGGG
GTTTTATGA
Antitoxin
Download Length: 116 a.a. Molecular weight: 13206.97 Da Isoelectric Point: 4.8313
>AT115093 WP_000681609.1 NZ_CP034061:c18705-18358 [Shigella flexneri]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA
Download Length: 348 bp
>AT115093 NZ_CP034061:c18705-18358 [Shigella flexneri]
ATGAAACATTTAAAATATAAAGGATATTTAGGTACGGTTGAGCCGGATTTTGAAAATAATATCCTGTATGGAAAGCTGGC
ATTTATTCGTGATCTGGTGACTTATGAGGCAGAAACATTGGCTGACCTGGAACGGGAATTTAAAACATCGGTTGATTTGT
ATTTACAGTCCTGTGTGGAGGACGGAAAGGAGCCTGACACGCCCTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAG
CTGCATCGTCGGGTCGCTGAAATGGCGATGGAAGAAGATTTGTCGCTGAATGCCTTTGTCAATAAGGCACTGGAAAAAGA
AGTCAGTCATCATCGCGCAGGGGCTTAA
ATGAAACATTTAAAATATAAAGGATATTTAGGTACGGTTGAGCCGGATTTTGAAAATAATATCCTGTATGGAAAGCTGGC
ATTTATTCGTGATCTGGTGACTTATGAGGCAGAAACATTGGCTGACCTGGAACGGGAATTTAAAACATCGGTTGATTTGT
ATTTACAGTCCTGTGTGGAGGACGGAAAGGAGCCTGACACGCCCTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAG
CTGCATCGTCGGGTCGCTGAAATGGCGATGGAAGAAGATTTGTCGCTGAATGCCTTTGTCAATAAGGCACTGGAAAAAGA
AGTCAGTCATCATCGCGCAGGGGCTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1I9ZJC5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1I9ZJ49 |