Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 3077634..3078429 | Replicon | chromosome |
| Accession | NZ_CP034060 | ||
| Organism | Shigella flexneri strain FDAARGOS_535 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | Q93EZ7 |
| Locus tag | EGX88_RS17250 | Protein ID | WP_000854917.1 |
| Coordinates | 3077634..3078008 (-) | Length | 125 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | D2ACA1 |
| Locus tag | EGX88_RS17255 | Protein ID | WP_001280958.1 |
| Coordinates | 3078055..3078429 (-) | Length | 125 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EGX88_RS17215 (3073243) | 3073243..3074181 | - | 939 | Protein_3012 | glyoxylate/hydroxypyruvate reductase GhrA | - |
| EGX88_RS17225 (3074653) | 3074653..3074934 | - | 282 | Protein_3013 | DUF4942 domain-containing protein | - |
| EGX88_RS17230 (3075216) | 3075216..3076220 | - | 1005 | WP_001179707.1 | IS110-like element ISSfl8 family transposase | - |
| EGX88_RS17235 (3076292) | 3076292..3076852 | - | 561 | Protein_3015 | DUF4942 domain-containing protein | - |
| EGX88_RS17240 (3076937) | 3076937..3077134 | - | 198 | WP_000839282.1 | DUF957 domain-containing protein | - |
| EGX88_RS17245 (3077146) | 3077146..3077637 | - | 492 | WP_000976828.1 | DUF5983 family protein | - |
| EGX88_RS17250 (3077634) | 3077634..3078008 | - | 375 | WP_000854917.1 | TA system toxin CbtA family protein | Toxin |
| EGX88_RS17255 (3078055) | 3078055..3078429 | - | 375 | WP_001280958.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| EGX88_RS17260 (3078592) | 3078592..3078813 | - | 222 | WP_001220314.1 | DUF987 domain-containing protein | - |
| EGX88_RS17265 (3078876) | 3078876..3079352 | - | 477 | WP_005011624.1 | RadC family protein | - |
| EGX88_RS17270 (3079368) | 3079368..3079841 | - | 474 | WP_005011597.1 | antirestriction protein | - |
| EGX88_RS17280 (3080183) | 3080183..3081004 | - | 822 | WP_001234584.1 | DUF932 domain-containing protein | - |
| EGX88_RS17285 (3081104) | 3081104..3081277 | - | 174 | WP_000088744.1 | DUF905 family protein | - |
| EGX88_RS26245 (3081397) | 3081397..3081660 | - | 264 | WP_000466628.1 | DNA polymerase III subunit gamma/tau | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | csgD / csgE / csgF / csgG | 3068929..3099918 | 30989 | |
| - | flank | IS/Tn | - | - | 3075216..3076220 | 1004 | |
| - | flank | IS/Tn | - | - | 3081843..3082346 | 503 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 125 a.a. Molecular weight: 14145.22 Da Isoelectric Point: 7.7761
>T115088 WP_000854917.1 NZ_CP034060:c3078008-3077634 [Shigella flexneri]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
Download Length: 375 bp
>T115088 NZ_CP034060:c3078008-3077634 [Shigella flexneri]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGTCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGTCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA
Antitoxin
Download Length: 125 a.a. Molecular weight: 13793.62 Da Isoelectric Point: 6.4651
>AT115088 WP_001280958.1 NZ_CP034060:c3078429-3078055 [Shigella flexneri]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
Download Length: 375 bp
>AT115088 NZ_CP034060:c3078429-3078055 [Shigella flexneri]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGTATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGTATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q93EZ7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0K4PAV2 |