Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1199151..1199805 Replicon chromosome
Accession NZ_CP034060
Organism Shigella flexneri strain FDAARGOS_535

Toxin (Protein)


Gene name cptA Uniprot ID Q0T101
Locus tag EGX88_RS07420 Protein ID WP_000244767.1
Coordinates 1199398..1199805 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID Q0T100
Locus tag EGX88_RS07415 Protein ID WP_000354044.1
Coordinates 1199151..1199417 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGX88_RS07390 (1194322) 1194322..1195064 + 743 Protein_1132 SDR family oxidoreductase -
EGX88_RS07395 (1195121) 1195121..1196554 - 1434 WP_005076748.1 6-phospho-beta-glucosidase BglA -
EGX88_RS07400 (1196599) 1196599..1196909 + 311 Protein_1134 N(4)-acetylcytidine aminohydrolase -
EGX88_RS07405 (1197073) 1197073..1197732 + 660 WP_000250274.1 hemolysin III family protein -
EGX88_RS07410 (1197928) 1197928..1198908 - 981 WP_000886065.1 tRNA-modifying protein YgfZ -
EGX88_RS07415 (1199151) 1199151..1199417 + 267 WP_000354044.1 FAD assembly factor SdhE Antitoxin
EGX88_RS07420 (1199398) 1199398..1199805 + 408 WP_000244767.1 protein YgfX Toxin
EGX88_RS07425 (1199845) 1199845..1200366 - 522 WP_001055867.1 flavodoxin FldB -
EGX88_RS07430 (1200478) 1200478..1201374 + 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
EGX88_RS07435 (1201399) 1201399..1202109 + 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EGX88_RS07440 (1202115) 1202115..1203848 + 1734 WP_000813187.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15978.91 Da        Isoelectric Point: 10.9373

>T115074 WP_000244767.1 NZ_CP034060:1199398-1199805 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T115074 NZ_CP034060:1199398-1199805 [Shigella flexneri]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGTGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10529.09 Da        Isoelectric Point: 5.1599

>AT115074 WP_000354044.1 NZ_CP034060:1199151-1199417 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT115074 NZ_CP034060:1199151-1199417 [Shigella flexneri]
ATGGACATTAACAACAAAGCCCGAATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATAATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TIU0

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TIU2

References