Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 78672..79231 | Replicon | plasmid unnamed1 |
| Accession | NZ_CP034059 | ||
| Organism | Shigella flexneri strain FDAARGOS_535 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | D2AJT2 |
| Locus tag | EGX88_RS00565 | Protein ID | WP_000421262.1 |
| Coordinates | 78672..78947 (-) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | - |
| Locus tag | EGX88_RS00570 | Protein ID | WP_024260696.1 |
| Coordinates | 78947..79231 (-) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EGX88_RS00535 (EGX88_00535) | 75548..75655 | - | 108 | WP_023592908.1 | transposase domain-containing protein | - |
| EGX88_RS00540 (EGX88_00540) | 75679..75984 | - | 306 | WP_001159860.1 | type II toxin-antitoxin system toxin CcdB | - |
| EGX88_RS00545 (EGX88_00545) | 75986..76204 | - | 219 | WP_000813626.1 | type II toxin-antitoxin system antitoxin CcdA | - |
| EGX88_RS00550 (EGX88_00550) | 76740..77693 | - | 954 | Protein_99 | IS66 family transposase | - |
| EGX88_RS00555 (EGX88_00555) | 77749..78446 | + | 698 | WP_223368647.1 | IS1 family transposase | - |
| EGX88_RS00565 (EGX88_00565) | 78672..78947 | - | 276 | WP_000421262.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| EGX88_RS00570 (EGX88_00570) | 78947..79231 | - | 285 | WP_024260696.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| EGX88_RS00580 (EGX88_00580) | 79683..79934 | + | 252 | WP_001381727.1 | transporter | - |
| EGX88_RS00585 (EGX88_00585) | 79984..80193 | + | 210 | Protein_104 | peptidoglycan-binding protein | - |
| EGX88_RS00590 (EGX88_00590) | 80293..80478 | - | 186 | Protein_105 | ATP-binding protein | - |
| EGX88_RS00595 (EGX88_00595) | 80667..82050 | - | 1384 | Protein_106 | IS66 family transposase | - |
| EGX88_RS00600 (EGX88_00600) | 82047..82397 | - | 351 | WP_005061851.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| EGX88_RS00605 (EGX88_00605) | 82394..83068 | - | 675 | WP_004967157.1 | IS66-like element accessory protein TnpA | - |
| EGX88_RS00610 (EGX88_00610) | 83148..84186 | - | 1039 | Protein_109 | IS3 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | ospC2 / ipgB2 / ospD1 / ospF / ospD2 / ospB / icsP/sopA / nleE / ospE1 / ipaH1.4 / ospG / ipaH9.8 / ospI / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ospC1 / ospD3/senA / ipaH4.5 / ipaH7.8 / ospE2 / ipaH2.5 | 1..217451 | 217451 | |
| - | inside | IScluster/Tn | - | ospG / ipaH9.8 / ospI | 69111..90608 | 21497 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10766.48 Da Isoelectric Point: 9.9324
>T115070 WP_000421262.1 NZ_CP034059:c78947-78672 [Shigella flexneri]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T115070 NZ_CP034059:c78947-78672 [Shigella flexneri]
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAGAACAGGTAG
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAGAACAGGTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10513.93 Da Isoelectric Point: 6.2150
>AT115070 WP_024260696.1 NZ_CP034059:c79231-78947 [Shigella flexneri]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSW
ADSLSTDHPLPVPR
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSW
ADSLSTDHPLPVPR
Download Length: 285 bp
>AT115070 NZ_CP034059:c79231-78947 [Shigella flexneri]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGTCCTGG
GCGGACAGCCTCAGTACTGACCATCCGTTACCGGTGCCACGCTGA
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGTCCTGG
GCGGACAGCCTCAGTACTGACCATCCGTTACCGGTGCCACGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|