Detailed information of TA system TA115061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 319775..320445
• Replicon: plasmid unnamed1
• Accession: NZ_CP034046
• Organism: Klebsiella pneumoniae strain KP_NORM_BLD_2014_104014

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: EHF42_RS27950
• Protein ID: WP_004213072.1
• Coordinates: 319775..320218 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: EHF42_RS27955
• Protein ID: WP_004213073.1
• Coordinates: 320215..320445 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHF42_RS27900	315187..315462	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
EHF42_RS27905	315525..316016	+	492	WP_011251327.1	DM13 domain-containing protein	-
EHF42_RS27910	316065..316985	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
EHF42_RS27920	317076..317482	+	407	Protein_337	GAF domain-containing protein	-
EHF42_RS30575	317997..318631	-	635	Protein_338	response regulator	-
EHF42_RS27940	319048..319341	+	294	Protein_339	transposase	-
EHF42_RS27945	319345..319761	+	417	Protein_340	restriction endonuclease	-
EHF42_RS27950	319775..320218	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
EHF42_RS27955	320215..320445	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EHF42_RS27960	321053..322186	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
EHF42_RS27965	322202..322495	+	294	WP_004213076.1	hypothetical protein	-
EHF42_RS27970	322485..322691	-	207	WP_004213077.1	hypothetical protein	-
EHF42_RS27975	323043..323333	+	291	WP_004213078.1	hypothetical protein	-
EHF42_RS27980	323323..324222	+	900	WP_004225022.1	nucleotide-binding protein	-
EHF42_RS27985	324347..325092	-	746	Protein_348	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IIa / blaTEM-1B / blaCTX-M-15 / aph(3')-Ia / dfrA1 / blaSHV-5 / sul1 / qacE / ant(3'')-Ia	iucA / iucB / iucC / iucD / iutA / rmpA	1..345775	345775
	-	flank	IS/Tn	-	-	325226..326020	794

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T115061 WP_004213072.1 NZ_CP034046:c320218-319775 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T115061 NZ_CP034046:c320218-319775 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT115061 WP_004213073.1 NZ_CP034046:c320445-320215 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT115061 NZ_CP034046:c320445-320215 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620