Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 278766..279676 Replicon plasmid unnamed1
Accession NZ_CP034046
Organism Klebsiella pneumoniae strain KP_NORM_BLD_2014_104014

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag EHF42_RS27685 Protein ID WP_004026354.1
Coordinates 278766..279236 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag EHF42_RS27690 Protein ID WP_004026357.1
Coordinates 279233..279676 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EHF42_RS27650 274051..274281 + 231 WP_011154571.1 hypothetical protein -
EHF42_RS27655 274564..274680 - 117 Protein_288 Tn3 family transposase -
EHF42_RS27660 274684..274869 + 186 WP_045145446.1 hypothetical protein -
EHF42_RS27665 274866..275345 + 480 WP_004212799.1 hypothetical protein -
EHF42_RS27670 275342..276181 + 840 WP_004212797.1 aldo/keto reductase -
EHF42_RS27675 276375..277379 + 1005 WP_000427623.1 IS110-like element IS4321 family transposase -
EHF42_RS27680 277730..278427 - 698 Protein_293 IS1 family transposase -
EHF42_RS27685 278766..279236 - 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
EHF42_RS27690 279233..279676 - 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
EHF42_RS27695 279777..281048 - 1272 WP_004181894.1 translesion error-prone DNA polymerase V subunit UmuC -
EHF42_RS27700 281048..281470 - 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
EHF42_RS27705 281778..283004 - 1227 Protein_298 transposase -
EHF42_RS27710 283046..283468 + 423 WP_101421878.1 IS200/IS605 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(3)-IIa / blaTEM-1B / blaCTX-M-15 / aph(3')-Ia / dfrA1 / blaSHV-5 / sul1 / qacE / ant(3'')-Ia iucA / iucB / iucC / iucD / iutA / rmpA 1..345775 345775
- inside IScluster/Tn - - 276375..283468 7093


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T115060 WP_004026354.1 NZ_CP034046:c279236-278766 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T115060 NZ_CP034046:c279236-278766 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACCAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT115060 WP_004026357.1 NZ_CP034046:c279676-279233 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT115060 NZ_CP034046:c279676-279233 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACGGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References