Detailed information of TA system TA115040

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 35946..36856
• Replicon: plasmid p1
• Accession: NZ_CP034040
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain CRK0298

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: C5X33_RS27810
• Protein ID: WP_080917295.1
• Coordinates: 36386..36856 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: C5X33_RS27805
• Protein ID: WP_004026357.1
• Coordinates: 35946..36389 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5X33_RS27780	31467..31934	+	468	Protein_41	transposase	-
C5X33_RS27790	32579..34066	+	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-
C5X33_RS27795	34152..34574	+	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
C5X33_RS27800	34574..35845	+	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
C5X33_RS27805	35946..36389	+	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
C5X33_RS27810	36386..36856	+	471	WP_080917295.1	RES family NAD+ phosphorylase	Toxin
C5X33_RS27825	38112..38372	-	261	WP_032719456.1	hypothetical protein	-
C5X33_RS27830	39036..40115	-	1080	WP_085844512.1	IS481-like element ISKpn28 family transposase	-
C5X33_RS27835	40187..40348	+	162	WP_004118124.1	type I toxin-antitoxin system Hok family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	qnrS1 / blaTEM-1B / catA1	-	1..213351	213351
	-	flank	IS/Tn	-	-	31416..31934	518
	-	flank	IS/Tn	-	-	39036..40115	1079

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17601.88 Da
• Isoelectric Point: 4.5152

>T115040 WP_080917295.1 NZ_CP034040:36386-36856 [Klebsiella pneumoniae subsp. pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKVQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T115040 NZ_CP034040:36386-36856 [Klebsiella pneumoniae subsp. pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTTACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATTGTGCAGAAGGTTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT115040 WP_004026357.1 NZ_CP034040:35946-36389 [Klebsiella pneumoniae subsp. pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT115040 NZ_CP034040:35946-36389 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGTCAAATCAATGAAGGCCTGGATGGGAAGGTGGCGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACTTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAATGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACGGGAGCGTATGAGG
TCATTAAGCTGATTACACGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A6P1V3Q9